Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis

James D. Firth, Daisuke Ekuni, Koichiro Irie, Takaaki Tomofuji, Manabu Morita, Edward E. Putnins

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aim: Lipopolysaccharide is a bacterial virulence factor implicated in chronic periodontitis, which may penetrate the junctional epithelial barrier and basement membrane to insult underlying stroma. We sought to identify lipopolysaccharide-induced global gene expression changes responsible for signalling between stroma and epithelium during disease onset. Materials and Methods Using a rat lipopolysaccharide periodontitis model, junctional epithelium and underlying stromal tissue were separately collected from healthy and diseased animals by laser-capture microdissection and subject to gene expression microarray analysis. Key gene products identified were validated in gingival epithelial and fibroblast cell cultures. Results: Global gene expression patterns distinguishing health versus disease were found in and between both tissue types. In stroma, the most significantly altered gene ontology function group (Z ≥ 4.00) was cytokines, containing most significantly (±2-fold; p <0.05) upregulated genes amphiregulin, IL1-β and Fas ligand, all positive, diffusible modulators of the epithelial growth factor receptor pathway. In epithelium, the most significant changes were in downregulated FOS-related antigen-1 gene, somatostatin receptor-2 gene and mucin-4 gene, all negative modulators of the epithelial growth factor receptor pathway. Conclusion: These results establish a periodontitis model for studying gene product interactions and suggests that the onset of junctional epithelial disease hyperproliferation involves a concerted stromal-epithelial signalling axis.

Original languageEnglish
Pages (from-to)8-17
Number of pages10
JournalJournal of Clinical Periodontology
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 2013

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Chronic Periodontitis
Lipopolysaccharides
Genes
Growth Factor Receptors
Periodontitis
Gene Expression
Mucin-4
Epithelium
Epithelial Attachment
Laser Capture Microdissection
Animal Diseases
Gene Ontology
Fas Ligand Protein
Virulence Factors
Microarray Analysis
Basement Membrane
Down-Regulation
Cell Culture Techniques
Fibroblasts
Epithelial Cells

Keywords

  • bioinformatics
  • epithelia
  • fibroblast
  • inflammation
  • lipopolysaccharide
  • signalling

ASJC Scopus subject areas

  • Periodontics

Cite this

Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis. / Firth, James D.; Ekuni, Daisuke; Irie, Koichiro; Tomofuji, Takaaki; Morita, Manabu; Putnins, Edward E.

In: Journal of Clinical Periodontology, Vol. 40, No. 1, 01.2013, p. 8-17.

Research output: Contribution to journalArticle

Firth, James D. ; Ekuni, Daisuke ; Irie, Koichiro ; Tomofuji, Takaaki ; Morita, Manabu ; Putnins, Edward E. / Lipopolysaccharide induces a stromal-epithelial signalling axis in a rat model of chronic periodontitis. In: Journal of Clinical Periodontology. 2013 ; Vol. 40, No. 1. pp. 8-17.
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