Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants

Isamu Sakurai, Jian Ren Shen, Jing Leng, Shunsuke Ohashi, Masami Kobayashi, Hajime Wada

    Research output: Contribution to journalArticlepeer-review

    89 Citations (Scopus)

    Abstract

    Lipids in dimeric photosystem II complexes prepared from two species of cyanobacteria, Thermosynechococcus vulcanus and Synechocystis sp. PCC6803, and two higher plants, spinach and rice, were analyzed to determine how many lipid molecules and what class of lipids are present in the photosystem II complexes. It was estimated that 27, 20, 8, and 7 lipid molecules per monomer are bound to the dimeric photosystem II complexes of T. vulcanus, Synechocystis, spinach, and rice, respectively. In each of the organisms, the lipid composition of the photosystem II complexes was quite different from that of the thylakoid membranes used for preparation of the complexes. The content of phosphatidylglycerol in the photosystem II complexes of each organism was much higher than that in the thylakoid membranes. Phospholipase A2 treatment of the photosystem II complexes of Synechocystis that degraded phosphatidylglycerol resulted in impairment of QB-mediated but not QA-mediated electron transport. These findings suggest that phosphatidylglycerol plays important roles in the electron transport at the QB-binding site in photosystem II complexes.

    Original languageEnglish
    Pages (from-to)201-209
    Number of pages9
    JournalJournal of biochemistry
    Volume140
    Issue number2
    DOIs
    Publication statusPublished - Aug 2006

    Keywords

    • Lipid
    • Phosphatidylglycerol
    • Photosynthesis
    • Photosystem II complex
    • Thylakoid membrane

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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