Lipid profiles of Helicobacter pylori and Helicobacter mustelae grown in serum-supplemented and serum-free media.

M. Haque, Y. Hirai, Kenji Yokota, K. Oguma

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Many of Helicobacter species have been found to have novel cholesteryl glucosides (CGs). To study the biosynthetic mechanism of CGs, the lipid profiles of H. pylori and H. mustelae grown in serum-supplemented and cholesterol-restricted serum-free media were investigated. In contrast to the serum-supplemented state, helicobacters had less CGs in the serum-free state; a trace amount of CGs and no CG was detected in H. pylori and H. mustelae, respectively. The proportion of total and individual phospholipid also showed significant alteration. Unknown lipids which did not contain phosphate and sugar were detected in the serum-free state, but not in the serum-supplemented state. The CGs were found to be distributed mainly in the membrane fractions, and one of the unknown lipids was found exclusively in the cytosol fraction. Based on these data, it is apparent that the CGs of helicobacters are synthesized by de novo uptake of cholesterol from the media. The unknown lipids detected in the serum-free state may be storage lipids, appearing in response to depletion of nutrients, especially cholesterol, or other factors in the media.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalActa Medica Okayama
Volume49
Issue number4
Publication statusPublished - Aug 1995

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Helicobacter mustelae
Serum-Free Culture Media
Helicobacter pylori
Lipids
Helicobacter
Serum
Cholesterol
Sugar Phosphates
cholesteryl glucoside
Cytosol
Nutrients
Phospholipids
Membranes
Food

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lipid profiles of Helicobacter pylori and Helicobacter mustelae grown in serum-supplemented and serum-free media. / Haque, M.; Hirai, Y.; Yokota, Kenji; Oguma, K.

In: Acta Medica Okayama, Vol. 49, No. 4, 08.1995, p. 205-211.

Research output: Contribution to journalArticle

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