Lipid bilayers at the gel interface for single ion channel recordings

Toru Ide, Toshihide Kobayashi, Minako Hirano

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Single-channel recording using artificial lipid bilayers is along with the patch-clamp technique a very powerful tool to physiologically and pharmacologically study ion channels. It is particularly advantageous in studying channels that are technically difficult to access with a patch pipet. However, the fragility of the bilayers and the difficulty to incorporate ion channels into them significantly compromises measurement efficiency. We have developed a novel method for forming artificial lipid bilayers on a hydrogel surface that significantly improves the measurement efficiency. Bilayers formed almost instantly (<1 s) and were able to incorporate various types of ion channel proteins within a short time (<30 s) enabling multichannel measurements. These results indicate that this method can potentially be applied to developing high-throughput screening devices for drug design.

Original languageEnglish
Pages (from-to)7792-7795
Number of pages4
JournalAnalytical Chemistry
Volume80
Issue number20
DOIs
Publication statusPublished - Oct 15 2008
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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