Lipid bilayers at gel/gel interface for ion channel recordings

Minako Hirano, Toshihide Kobayashi, Toru Ide

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have developed a practical method to produce durable artificial lipid bilayers using a hydrogel-hydrogel interface for ion channel measurements. Bilayers were formed by forcing a hydrogel-bead into contact with the hydrogel layer (hydrogel plate) in a lipid solution. The immediate formation of a bilayer was observed (<1 s). This allows channel recordings to be repeated more easily and quickly as compared to conventional methods. Currents of various types of channel such as gramicidin, hemolysin and BK-channel have been recorded. Our channel property results mirrored those of other techniques and were reproducible. Hydrogel solutions containing gramicidin were extremely stable and could be used months after preparation for bilayer experiments.

Original languageEnglish
Pages (from-to)130-133
Number of pages4
Journale-Journal of Surface Science and Nanotechnology
Volume6
DOIs
Publication statusPublished - May 31 2008
Externally publishedYes

Fingerprint

Lipid bilayers
Hydrogel
Lipid Bilayers
Ion Channels
Hydrogels
lipids
Gels
recording
gels
Ions
Gramicidin
beads
Large-Conductance Calcium-Activated Potassium Channels
Hemolysin Proteins
Lipids
preparation
Experiments

Keywords

  • Biological molecules-proteins
  • Biomedical engineering
  • Biophysics
  • Biosensing and devices
  • Ion channel
  • Medical physics
  • Single channel recording

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Materials Science (miscellaneous)

Cite this

Lipid bilayers at gel/gel interface for ion channel recordings. / Hirano, Minako; Kobayashi, Toshihide; Ide, Toru.

In: e-Journal of Surface Science and Nanotechnology, Vol. 6, 31.05.2008, p. 130-133.

Research output: Contribution to journalArticle

@article{1ec797e6133745bb907aefda13287879,
title = "Lipid bilayers at gel/gel interface for ion channel recordings",
abstract = "We have developed a practical method to produce durable artificial lipid bilayers using a hydrogel-hydrogel interface for ion channel measurements. Bilayers were formed by forcing a hydrogel-bead into contact with the hydrogel layer (hydrogel plate) in a lipid solution. The immediate formation of a bilayer was observed (<1 s). This allows channel recordings to be repeated more easily and quickly as compared to conventional methods. Currents of various types of channel such as gramicidin, hemolysin and BK-channel have been recorded. Our channel property results mirrored those of other techniques and were reproducible. Hydrogel solutions containing gramicidin were extremely stable and could be used months after preparation for bilayer experiments.",
keywords = "Biological molecules-proteins, Biomedical engineering, Biophysics, Biosensing and devices, Ion channel, Medical physics, Single channel recording",
author = "Minako Hirano and Toshihide Kobayashi and Toru Ide",
year = "2008",
month = "5",
day = "31",
doi = "10.1380/ejssnt.2008.130",
language = "English",
volume = "6",
pages = "130--133",
journal = "e-Journal of Surface Science and Nanotechnology",
issn = "1348-0391",
publisher = "Surface Science Society of Japan",

}

TY - JOUR

T1 - Lipid bilayers at gel/gel interface for ion channel recordings

AU - Hirano, Minako

AU - Kobayashi, Toshihide

AU - Ide, Toru

PY - 2008/5/31

Y1 - 2008/5/31

N2 - We have developed a practical method to produce durable artificial lipid bilayers using a hydrogel-hydrogel interface for ion channel measurements. Bilayers were formed by forcing a hydrogel-bead into contact with the hydrogel layer (hydrogel plate) in a lipid solution. The immediate formation of a bilayer was observed (<1 s). This allows channel recordings to be repeated more easily and quickly as compared to conventional methods. Currents of various types of channel such as gramicidin, hemolysin and BK-channel have been recorded. Our channel property results mirrored those of other techniques and were reproducible. Hydrogel solutions containing gramicidin were extremely stable and could be used months after preparation for bilayer experiments.

AB - We have developed a practical method to produce durable artificial lipid bilayers using a hydrogel-hydrogel interface for ion channel measurements. Bilayers were formed by forcing a hydrogel-bead into contact with the hydrogel layer (hydrogel plate) in a lipid solution. The immediate formation of a bilayer was observed (<1 s). This allows channel recordings to be repeated more easily and quickly as compared to conventional methods. Currents of various types of channel such as gramicidin, hemolysin and BK-channel have been recorded. Our channel property results mirrored those of other techniques and were reproducible. Hydrogel solutions containing gramicidin were extremely stable and could be used months after preparation for bilayer experiments.

KW - Biological molecules-proteins

KW - Biomedical engineering

KW - Biophysics

KW - Biosensing and devices

KW - Ion channel

KW - Medical physics

KW - Single channel recording

UR - http://www.scopus.com/inward/record.url?scp=45949094516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45949094516&partnerID=8YFLogxK

U2 - 10.1380/ejssnt.2008.130

DO - 10.1380/ejssnt.2008.130

M3 - Article

AN - SCOPUS:45949094516

VL - 6

SP - 130

EP - 133

JO - e-Journal of Surface Science and Nanotechnology

JF - e-Journal of Surface Science and Nanotechnology

SN - 1348-0391

ER -