Limited differentiation to neurons and astroglia from neural stem cells in the cortex and striatum after ischemia/hypoxia in the neonatal rat brain

Tomoaki Ikeda, Masanori Iwai, Takeshi Hayashi, Isao Nagano, Mikio Shogi, Tsuyomu Ikenoue, Koji Abe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: We examined whether progenitor neural stem cells can differentiate successfully into mature neurons and astrocytes in a rat model of neonatal hypoxic-ischemic encephalopathy. Study design: Seven-day-old Wistar rats were subjected to hypoxic-ischemic stress. At days 5 to 7 after hypoxic-ischemic stress, 5-bromodeoxyuridine (an early marker of cell proliferation) was injected, and the brains were retrieved at 14, 28, and 42 days after hypoxic-ischemic stress. Immunohistochemical and immunofluorescent studies were carried out for 5-bromodeoxyuridine, neuronal nuclear antigen (a marker protein of matured neuron), and glial fibrillary acidic protein (a protein marker of mature astrocytes). Results: Only 1% of neuronal nuclear antigen-positive and 4.6% of glial fibrillary acidic protein-positive cells could be detected among the 5-bromodeoxyuridine-immunopositive cells in the peri-infarcted area of the cortex and the striatum, respectively, at 14 days after hypoxic-ischemic stress. There were no such double-staining cells at 28 and 42 days after hypoxic-ischemic stress. Conclusion: The intrinsic ability for neurologic self-repair was limited at the maturation step after hypoxic-ischemic stress in the neonatal rat brain.

Original languageEnglish
Pages (from-to)849-856
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume193
Issue number3
DOIs
Publication statusPublished - Sep 2005

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Neural Stem Cells
Bromodeoxyuridine
Astrocytes
Nuclear Antigens
Ischemia
Glial Fibrillary Acidic Protein
Neurons
Brain
Brain Hypoxia-Ischemia
Nervous System
Wistar Rats
Proteins
Cell Proliferation
Staining and Labeling
Hypoxia

Keywords

  • Differentiation
  • Hypoxic-ischemic encephalopathy
  • Neonatal rat
  • Neural stem cell

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynaecology

Cite this

Limited differentiation to neurons and astroglia from neural stem cells in the cortex and striatum after ischemia/hypoxia in the neonatal rat brain. / Ikeda, Tomoaki; Iwai, Masanori; Hayashi, Takeshi; Nagano, Isao; Shogi, Mikio; Ikenoue, Tsuyomu; Abe, Koji.

In: American Journal of Obstetrics and Gynecology, Vol. 193, No. 3, 09.2005, p. 849-856.

Research output: Contribution to journalArticle

Ikeda, Tomoaki ; Iwai, Masanori ; Hayashi, Takeshi ; Nagano, Isao ; Shogi, Mikio ; Ikenoue, Tsuyomu ; Abe, Koji. / Limited differentiation to neurons and astroglia from neural stem cells in the cortex and striatum after ischemia/hypoxia in the neonatal rat brain. In: American Journal of Obstetrics and Gynecology. 2005 ; Vol. 193, No. 3. pp. 849-856.
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AU - Abe, Koji

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