Limited differentiation to neurons and astroglia from neural stem cells in the cortex and striatum after ischemia/hypoxia in the neonatal rat brain

Tomoaki Ikeda, Masanori Iwai, Takeshi Hayashi, Isao Nagano, Mikio Shogi, Tsuyomu Ikenoue, Koji Abe

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: We examined whether progenitor neural stem cells can differentiate successfully into mature neurons and astrocytes in a rat model of neonatal hypoxic-ischemic encephalopathy. Study design: Seven-day-old Wistar rats were subjected to hypoxic-ischemic stress. At days 5 to 7 after hypoxic-ischemic stress, 5-bromodeoxyuridine (an early marker of cell proliferation) was injected, and the brains were retrieved at 14, 28, and 42 days after hypoxic-ischemic stress. Immunohistochemical and immunofluorescent studies were carried out for 5-bromodeoxyuridine, neuronal nuclear antigen (a marker protein of matured neuron), and glial fibrillary acidic protein (a protein marker of mature astrocytes). Results: Only 1% of neuronal nuclear antigen-positive and 4.6% of glial fibrillary acidic protein-positive cells could be detected among the 5-bromodeoxyuridine-immunopositive cells in the peri-infarcted area of the cortex and the striatum, respectively, at 14 days after hypoxic-ischemic stress. There were no such double-staining cells at 28 and 42 days after hypoxic-ischemic stress. Conclusion: The intrinsic ability for neurologic self-repair was limited at the maturation step after hypoxic-ischemic stress in the neonatal rat brain.

Original languageEnglish
Pages (from-to)849-856
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume193
Issue number3
DOIs
Publication statusPublished - Sep 2005

Keywords

  • Differentiation
  • Hypoxic-ischemic encephalopathy
  • Neonatal rat
  • Neural stem cell

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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