Light inhibition of shoot regeneration is regulated by endogenous abscisic acid level in calli derived from immature barley embryos

Kazuhide Rikiishi, Takakazu Matsuura, Yoko Ikeda, Masahiko Maekawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Shoot regeneration in calli derived from immature barley embryos is regulated by light conditions during the callus-induction period. Barley cultivars Kanto Nijo-5 (KN5) and K-3 (K3) showed lower efficiency of shoot regeneration in a 16-h photoperiod during callus-induction than those in continuous darkness, whereas shoot regeneration was enhanced in cultures under a 16-h photoperiod in Golden Promise (GP) and Lenins (LN). These cultivars were classified as photo-inhibition type (KN5 and K3) or photo-induction type (GP and LN) according to their response to light. Contents of endogenous plant hormones were determined in calli cultured under a 16-h photoperiod and continuous darkness. In photo-inhibition type, higher accumulation of abscisic acid (ABA) was detected in calli cultured under a 16-h photoperiod, whereas calli showed lower levels of endogenous ABA in continuous darkness. However, cultivars of photo-induction type showed lower levels of ABA in calli cultured under both light conditions, similarly to photo-inhibition type in continuous darkness. Exogenous ABA inhibited the callus growth and shoot regeneration independent of light conditions in all cultivars. In photo-inhibition type, lower levels of endogenous ABA induced by ABA biosynthesis inhibitor, fluridone, reduced the photo-inhibition of shoot regeneration. Expression of ABA biosynthesis gene, HvNCED1, in calli was regulated by the light conditions. Higher expression was observed in calli cultured under a 16-h photoperiod. These results indicate that ABA biosynthesis could be activated through the higher expression of HvNCED1 in a 16-h photoperiod and that the higher accumulations of ABA inhibit shoot regeneration in the photo-inhibition type cultivars.

Original languageEnglish
Article number0145242
JournalPLoS One
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Abscisic Acid
Bony Callus
Hordeum
abscisic acid
Regeneration
callus
Embryonic Structures
immatures
barley
Photoperiod
Light
photoinhibition
shoots
photoperiod
Darkness
Biosynthesis
cultivars
biosynthesis
fluridone
Plant Growth Regulators

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Light inhibition of shoot regeneration is regulated by endogenous abscisic acid level in calli derived from immature barley embryos. / Rikiishi, Kazuhide; Matsuura, Takakazu; Ikeda, Yoko; Maekawa, Masahiko.

In: PLoS One, Vol. 10, No. 12, 0145242, 01.12.2015.

Research output: Contribution to journalArticle

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