Light-induced Fourier transform infrared spectrum of the cation radical P680+

Suleyman Allakhverdiev, A. Ahmed, H. A. Tajmir-Riahi, V. V. Klimov, R. Carpentier

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The structure of the primary electron donor of photosystem II, P680, is still under debate. It is not decided if it is composed of a chlorophyll (Chl) monomer or dimer. In this study, Fourier transform infrared (FTIR) spectroscopy was used to analyze the changes in the vibration modes occurring upon photooxidation of P680 in a Mn-depleted PS II preparation. It is demonstrated that illumination of the above in the presence of artificial electron acceptors results in a light-minus-dark absorbance change typical of the formation ofP680+. The light-minus-dark difference FTIR spectrum obtained under similar conditions is characterized by two negative peaks located at 1694 and 1652 or 1626 cm-1 that can be assigned to the 9-keto groups of the P680 Chl, the latter band being indicative of a strongly associated group. These vibrations are shifted to 1714 and 1676 cm-1, respectively, in the positive features of the difference spectrum attributed to P680+. The occurrence of two pairs of bands attributed to 9-keto groups is discussed in terms of P680 being formed of a Chl dimer.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalFEBS Letters
Volume339
Issue number1-2
DOIs
Publication statusPublished - Feb 14 1994
Externally publishedYes

Fingerprint

Fourier Analysis
Chlorophyll
Cations
Fourier transforms
Vibration
Infrared radiation
Light
Dimers
Electrons
Photosystem II Protein Complex
Photooxidation
Fourier Transform Infrared Spectroscopy
Lighting
Monomers

Keywords

  • Chlorophyll
  • Fourier transform infrared spectroscopy
  • P680
  • Photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Allakhverdiev, S., Ahmed, A., Tajmir-Riahi, H. A., Klimov, V. V., & Carpentier, R. (1994). Light-induced Fourier transform infrared spectrum of the cation radical P680+. FEBS Letters, 339(1-2), 151-154. https://doi.org/10.1016/0014-5793(94)80404-4

Light-induced Fourier transform infrared spectrum of the cation radical P680+. / Allakhverdiev, Suleyman; Ahmed, A.; Tajmir-Riahi, H. A.; Klimov, V. V.; Carpentier, R.

In: FEBS Letters, Vol. 339, No. 1-2, 14.02.1994, p. 151-154.

Research output: Contribution to journalArticle

Allakhverdiev, S, Ahmed, A, Tajmir-Riahi, HA, Klimov, VV & Carpentier, R 1994, 'Light-induced Fourier transform infrared spectrum of the cation radical P680+', FEBS Letters, vol. 339, no. 1-2, pp. 151-154. https://doi.org/10.1016/0014-5793(94)80404-4
Allakhverdiev S, Ahmed A, Tajmir-Riahi HA, Klimov VV, Carpentier R. Light-induced Fourier transform infrared spectrum of the cation radical P680+. FEBS Letters. 1994 Feb 14;339(1-2):151-154. https://doi.org/10.1016/0014-5793(94)80404-4
Allakhverdiev, Suleyman ; Ahmed, A. ; Tajmir-Riahi, H. A. ; Klimov, V. V. ; Carpentier, R. / Light-induced Fourier transform infrared spectrum of the cation radical P680+. In: FEBS Letters. 1994 ; Vol. 339, No. 1-2. pp. 151-154.
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