Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis

Ryo Nagao; Tatsuya Tomo; Rei Narikawa; Isao Enami; Masahiko Ikeuchi

doi:10.1016/j.febslet.2013.02.050

Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis

Ryo Nagao, Tatsuya Tomo, Rei Narikawa, Isao Enami, Masahiko Ikeuchi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.

Original language	English
Pages (from-to)	1340-1345
Number of pages	6
Journal	FEBS Letters
Volume	587
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2013.02.050
Publication status	Published - May 2 2013
Externally published	Yes

Keywords

FCP
PSII monomer
RC47
Turnover

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis",

abstract = "Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.",

keywords = "FCP, PSII monomer, RC47, Turnover",

author = "Ryo Nagao and Tatsuya Tomo and Rei Narikawa and Isao Enami and Masahiko Ikeuchi",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan (Nos. 21570038 , 22370017 , 24370025 to T.T.; and Nos. 17657013 , 20370018 , 21657013 to M.I.), by a Research Fellowship to R.N. from the Japan Society for the Promotion of Science, by a grant from JST PRESTO to T.T., by a grant from the Australian Research Council{\textquoteright}s Discovery Projects funding scheme (project number DP12101360) to T.T., by a grant from JST CREST to M.I., and by the Global COE program (From the Earth to “Earths”) to M.I.",

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doi = "10.1016/j.febslet.2013.02.050",

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AU - Nagao, Ryo

AU - Tomo, Tatsuya

AU - Narikawa, Rei

AU - Enami, Isao

AU - Ikeuchi, Masahiko

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan (Nos. 21570038 , 22370017 , 24370025 to T.T.; and Nos. 17657013 , 20370018 , 21657013 to M.I.), by a Research Fellowship to R.N. from the Japan Society for the Promotion of Science, by a grant from JST PRESTO to T.T., by a grant from the Australian Research Council’s Discovery Projects funding scheme (project number DP12101360) to T.T., by a grant from JST CREST to M.I., and by the Global COE program (From the Earth to “Earths”) to M.I.

PY - 2013/5/2

Y1 - 2013/5/2

N2 - Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.

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