Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis

Ryo Nagao; Tatsuya Tomo; Rei Narikawa; Isao Enami; Masahiko Ikeuchi

doi:10.1016/j.febslet.2013.02.050

Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis

Ryo Nagao, Tatsuya Tomo, Rei Narikawa, Isao Enami, Masahiko Ikeuchi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.

Original language	English
Pages (from-to)	1340-1345
Number of pages	6
Journal	FEBS Letters
Volume	587
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2013.02.050
Publication status	Published - May 2 2013
Externally published	Yes

Keywords

FCP
PSII monomer
RC47
Turnover

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2013.02.050

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title = "Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis",

abstract = "Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.",

keywords = "FCP, PSII monomer, RC47, Turnover",

author = "Ryo Nagao and Tatsuya Tomo and Rei Narikawa and Isao Enami and Masahiko Ikeuchi",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan (Nos. 21570038 , 22370017 , 24370025 to T.T.; and Nos. 17657013 , 20370018 , 21657013 to M.I.), by a Research Fellowship to R.N. from the Japan Society for the Promotion of Science, by a grant from JST PRESTO to T.T., by a grant from the Australian Research Council{\textquoteright}s Discovery Projects funding scheme (project number DP12101360) to T.T., by a grant from JST CREST to M.I., and by the Global COE program (From the Earth to “Earths”) to M.I.",

year = "2013",

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doi = "10.1016/j.febslet.2013.02.050",

language = "English",

volume = "587",

pages = "1340--1345",

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T1 - Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis

AU - Nagao, Ryo

AU - Tomo, Tatsuya

AU - Narikawa, Rei

AU - Enami, Isao

AU - Ikeuchi, Masahiko

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan (Nos. 21570038 , 22370017 , 24370025 to T.T.; and Nos. 17657013 , 20370018 , 21657013 to M.I.), by a Research Fellowship to R.N. from the Japan Society for the Promotion of Science, by a grant from JST PRESTO to T.T., by a grant from the Australian Research Council’s Discovery Projects funding scheme (project number DP12101360) to T.T., by a grant from JST CREST to M.I., and by the Global COE program (From the Earth to “Earths”) to M.I.

PY - 2013/5/2

Y1 - 2013/5/2

N2 - Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.

AB - Diatoms can survive for long periods in the dark. However, how biosynthesis of photosynthetic proteins contributes to survival in the dark is poorly understood. Using a radiolabeling technique, we examined whether de novo biosynthesis and assembly of photosynthetic proteins differs in light-adapted vs. dark-adapted marine diatoms (Chaetoceros gracilis). In light-adapted cells, D1 protein was heavily radiolabeled owing to rapid turnover of photosystem II (PSII). In dark-adapted cells (>24 h), the radiolabeling patterns of PSII components changed, but the PSII dimer still formed. Therefore, diatoms may regulate the biosynthesis of photosynthetic proteins for long-term survival in the dark.

KW - FCP

KW - PSII monomer

KW - RC47

KW - Turnover

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Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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