Abstract
Cold stress causes unsaturation of the membrane lipids. This leads to adjustment of the membrane fluidity, which is necessary for cold acclimation of cells. Here we demonstrate that the cold-induced accumulation of PUFAs in the cyanobacterium Synechocystis is light-dependent. The desA-/desD - mutant, that lacks the genes for Δ12 and Δ6 desaturases, is still able to adjust the fluidity of its membranes in spite of its inability to synthesize PUFAs and modulate the fatty acid composition of the membrane lipids under cold stress. The expression of cold-induced genes, which are controlled by the cold sensor histidine kinase Hik33, depends on the fluidity of cell membranes and it is regulated by light, though it does not require the activity of the photosynthetic apparatus. The expression of cold-induced genes, which are not controlled by Hik33, does not depend on the membrane fluidity or light. Thus, membrane fluidity determines the temperature dependence of the expression of cold-induced genes that are under control of the Hik33, which might be the sensor of changes in the membrane fluidity. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
| Original language | English |
|---|---|
| Pages (from-to) | 1352-1359 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - Bioenergetics |
| Volume | 1817 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 1 2012 |
| Externally published | Yes |
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Keywords
- Cold stress
- Fatty acid desaturase
- Gene expression
- Membrane fluidity
- Synechocystis
- Unsaturated fatty acid
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology
Cite this
Light-dependent cold-induced fatty acid unsaturation, changes in membrane fluidity, and alterations in gene expression in Synechocystis. / Mironov, Kirill S.; Sidorov, Roman A.; Trofimova, Marina S.; Bedbenov, Vladimir S.; Tsydendambaev, Vladimir D.; Allakhverdiev, Suleyman; Los, Dmitry A.
In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1817, No. 8, 01.08.2012, p. 1352-1359.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Light-dependent cold-induced fatty acid unsaturation, changes in membrane fluidity, and alterations in gene expression in Synechocystis
AU - Mironov, Kirill S.
AU - Sidorov, Roman A.
AU - Trofimova, Marina S.
AU - Bedbenov, Vladimir S.
AU - Tsydendambaev, Vladimir D.
AU - Allakhverdiev, Suleyman
AU - Los, Dmitry A.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - Cold stress causes unsaturation of the membrane lipids. This leads to adjustment of the membrane fluidity, which is necessary for cold acclimation of cells. Here we demonstrate that the cold-induced accumulation of PUFAs in the cyanobacterium Synechocystis is light-dependent. The desA-/desD - mutant, that lacks the genes for Δ12 and Δ6 desaturases, is still able to adjust the fluidity of its membranes in spite of its inability to synthesize PUFAs and modulate the fatty acid composition of the membrane lipids under cold stress. The expression of cold-induced genes, which are controlled by the cold sensor histidine kinase Hik33, depends on the fluidity of cell membranes and it is regulated by light, though it does not require the activity of the photosynthetic apparatus. The expression of cold-induced genes, which are not controlled by Hik33, does not depend on the membrane fluidity or light. Thus, membrane fluidity determines the temperature dependence of the expression of cold-induced genes that are under control of the Hik33, which might be the sensor of changes in the membrane fluidity. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
AB - Cold stress causes unsaturation of the membrane lipids. This leads to adjustment of the membrane fluidity, which is necessary for cold acclimation of cells. Here we demonstrate that the cold-induced accumulation of PUFAs in the cyanobacterium Synechocystis is light-dependent. The desA-/desD - mutant, that lacks the genes for Δ12 and Δ6 desaturases, is still able to adjust the fluidity of its membranes in spite of its inability to synthesize PUFAs and modulate the fatty acid composition of the membrane lipids under cold stress. The expression of cold-induced genes, which are controlled by the cold sensor histidine kinase Hik33, depends on the fluidity of cell membranes and it is regulated by light, though it does not require the activity of the photosynthetic apparatus. The expression of cold-induced genes, which are not controlled by Hik33, does not depend on the membrane fluidity or light. Thus, membrane fluidity determines the temperature dependence of the expression of cold-induced genes that are under control of the Hik33, which might be the sensor of changes in the membrane fluidity. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
KW - Cold stress
KW - Fatty acid desaturase
KW - Gene expression
KW - Membrane fluidity
KW - Synechocystis
KW - Unsaturated fatty acid
UR - http://www.scopus.com/inward/record.url?scp=84862226736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862226736&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2011.12.011
DO - 10.1016/j.bbabio.2011.12.011
M3 - Article
C2 - 22222355
AN - SCOPUS:84862226736
VL - 1817
SP - 1352
EP - 1359
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 8
ER -