Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos

Kazuhide Rikiishi, Takakazu Matsuura, Masahiko Maekawa, Kazuyoshi Takeda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Callus growth and shoot regeneration were examined in cultures of immature barley embryos incubated under various combinations of a week under a 16-h photoperiod and a week under continuous darkness. Incubation in darkness for four weeks, during which calli were formed, enhanced shoot regeneration in 'K-3' and 'Kanto Nijo-5', but inhibited it in 'Lenins'. 'K-3' and 'Kanto Nijo-5', incubated in darkness during the first two weeks of callus induction, followed by two weeks of a 16-h photoperiod (D2L2), showed a higher percentage of shoot regeneration than those incubated for two weeks with a 16-h photoperiod followed by two weeks of darkness (L2D2). Nevertheless, when the light conditions, D2L2 and L2D2, were combined into the same periods of a 16-h photoperiod and continuous darkness, except for the order, light conditions affected shoot regeneration differently. The early stage of callus induction seems to be sensitive to light. The expression ratios of the auxin-responsive gene (AUX/IAA) to the cytokinin-responsive gene (WPK4) were increased in continuous darkness in 'K-3' and 'Kanto Nijo-5'. The effects of light qualities (white, red, farred, and blue) on callus growth and shoot regeneration were also examined. Blue light inhibited shoot regeneration, as did white light, in 'K-3' and 'Kanto Nijo-5', and far-red light in 'Kanto Nijo-5'. Light probably controls shoot regeneration from calli by modifying cytokinin levels and/or response; blue light signals act in photo-inhibition of shoot regeneration in immature barley embryo culture.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalBreeding Science
Volume58
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Bony Callus
callus culture
Hordeum
Hordeum vulgare
Regeneration
Embryonic Structures
Darkness
immatures
barley
Light
callus
shoots
Photoperiod
photoperiod
Cytokinins
blue light
cytokinins
light quality
embryo culture
far-red light

Keywords

  • Barley (Hordeum vulgare L.)
  • Genotype
  • Immature embryo
  • Light control
  • Shoot regeneration

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos. / Rikiishi, Kazuhide; Matsuura, Takakazu; Maekawa, Masahiko; Takeda, Kazuyoshi.

In: Breeding Science, Vol. 58, No. 2, 2008, p. 129-135.

Research output: Contribution to journalArticle

Rikiishi, Kazuhide ; Matsuura, Takakazu ; Maekawa, Masahiko ; Takeda, Kazuyoshi. / Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos. In: Breeding Science. 2008 ; Vol. 58, No. 2. pp. 129-135.
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