Ligation of HLA class II molecules promotes sensitivity to CD95 (Fas antigen, APO-1)-mediated apoptosis

Tadashi Yoshino, Liu Cao, Ritsuo Nishiuchi, Yoshinobu Matsuo, Ichiro Yamadori, Eisaku Kondo, Norihiro Teramoto, Kazuhiko Hayashi, Kiyoshi Takahashi, Nobuhiro Kamikawaji, Tadaatsu Akagi

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Abstract

CD95 (Fas antigen/APO-1) is up-regulated in activated lymphocytes, and monoclonal antibody (mAb) to CD95 induces apoptosis. HLA class II molecules play a key role in antigen presentation, ligation of which induces signal transduction. We examined 18 lymphoid cell lines (15 B cell and 3 T cell lines) to investigate the effects of ligation of HLA class II molecules on CD95-mediated apoptosis. All of the five immature B cell lines were sensitive to anti-CD95 mAb, and ligation of HLA class II molecules promoted CD95-mediated apoptosis. In seven B-blastoid cell lines, two Burkitt lines were resistant to anti-CD95 mAb in spite of high expression of CD95. In three of five non-Burkitt B-blastoid lines, CD95-mediated apoptosis was augmented by treatment with anti-HLA class II mAb, while the other two lines lacking CD95 were resistant to anti-CD95 mAb. Three plasmacytic cell lines showed CD95-mediated apoptosis, but enhancement by anti-HLA class II mAb was slight in one cell line and was not observed in the other two lines. Out of three HLA class II antigen-positive T cell lines, CD95-mediated apoptosis was observed to some degree in one cell line but was not promoted by the treatment with anti-HLA class II mAb, and the other two cell lines were resistant to anti-CD95 mAb. Ligation of HLA class II molecules did not alter CD95 expression in the five cell lines examined, except Su-DHL-4 originated from a follicular lymphoma, which showed slight up-regulation. Taken together, ligation of HLA class II molecules apparently promotes CD95-mediated apoptosis in immature B cells and non-Burkitt B blasts. These findings highlight the role of HLA class II molecules in CD95-mediated apoptosis, which may facilitate rapid clearance of functionally useless cells from the immune system and might be involved in negative selection of B cells.

Original languageEnglish
Pages (from-to)2190-2194
Number of pages5
JournalEuropean Journal of Immunology
Volume25
Issue number8
DOIs
Publication statusPublished - Aug 1995

Fingerprint

CD95 Antigens
Ligation
Apoptosis
Cell Line
Monoclonal Antibodies
B-Lymphoid Precursor Cells
B-Lymphocytes
Lymphocytes
T-Lymphocytes
Follicular Lymphoma
Histocompatibility Antigens Class II
Antigen Presentation
HLA Antigens
Immune System
Signal Transduction
Up-Regulation

Keywords

  • APO-1
  • Apoptosis
  • CD95
  • Fas antigen
  • HLA class II

ASJC Scopus subject areas

  • Immunology

Cite this

Ligation of HLA class II molecules promotes sensitivity to CD95 (Fas antigen, APO-1)-mediated apoptosis. / Yoshino, Tadashi; Cao, Liu; Nishiuchi, Ritsuo; Matsuo, Yoshinobu; Yamadori, Ichiro; Kondo, Eisaku; Teramoto, Norihiro; Hayashi, Kazuhiko; Takahashi, Kiyoshi; Kamikawaji, Nobuhiro; Akagi, Tadaatsu.

In: European Journal of Immunology, Vol. 25, No. 8, 08.1995, p. 2190-2194.

Research output: Contribution to journalArticle

Yoshino, T, Cao, L, Nishiuchi, R, Matsuo, Y, Yamadori, I, Kondo, E, Teramoto, N, Hayashi, K, Takahashi, K, Kamikawaji, N & Akagi, T 1995, 'Ligation of HLA class II molecules promotes sensitivity to CD95 (Fas antigen, APO-1)-mediated apoptosis', European Journal of Immunology, vol. 25, no. 8, pp. 2190-2194. https://doi.org/10.1002/eji.1830250811
Yoshino, Tadashi ; Cao, Liu ; Nishiuchi, Ritsuo ; Matsuo, Yoshinobu ; Yamadori, Ichiro ; Kondo, Eisaku ; Teramoto, Norihiro ; Hayashi, Kazuhiko ; Takahashi, Kiyoshi ; Kamikawaji, Nobuhiro ; Akagi, Tadaatsu. / Ligation of HLA class II molecules promotes sensitivity to CD95 (Fas antigen, APO-1)-mediated apoptosis. In: European Journal of Immunology. 1995 ; Vol. 25, No. 8. pp. 2190-2194.
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AU - Yamadori, Ichiro

AU - Kondo, Eisaku

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AB - CD95 (Fas antigen/APO-1) is up-regulated in activated lymphocytes, and monoclonal antibody (mAb) to CD95 induces apoptosis. HLA class II molecules play a key role in antigen presentation, ligation of which induces signal transduction. We examined 18 lymphoid cell lines (15 B cell and 3 T cell lines) to investigate the effects of ligation of HLA class II molecules on CD95-mediated apoptosis. All of the five immature B cell lines were sensitive to anti-CD95 mAb, and ligation of HLA class II molecules promoted CD95-mediated apoptosis. In seven B-blastoid cell lines, two Burkitt lines were resistant to anti-CD95 mAb in spite of high expression of CD95. In three of five non-Burkitt B-blastoid lines, CD95-mediated apoptosis was augmented by treatment with anti-HLA class II mAb, while the other two lines lacking CD95 were resistant to anti-CD95 mAb. Three plasmacytic cell lines showed CD95-mediated apoptosis, but enhancement by anti-HLA class II mAb was slight in one cell line and was not observed in the other two lines. Out of three HLA class II antigen-positive T cell lines, CD95-mediated apoptosis was observed to some degree in one cell line but was not promoted by the treatment with anti-HLA class II mAb, and the other two cell lines were resistant to anti-CD95 mAb. Ligation of HLA class II molecules did not alter CD95 expression in the five cell lines examined, except Su-DHL-4 originated from a follicular lymphoma, which showed slight up-regulation. Taken together, ligation of HLA class II molecules apparently promotes CD95-mediated apoptosis in immature B cells and non-Burkitt B blasts. These findings highlight the role of HLA class II molecules in CD95-mediated apoptosis, which may facilitate rapid clearance of functionally useless cells from the immune system and might be involved in negative selection of B cells.

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