Ligand-binding properties of annexin from Caenorhabditis elegans (Annexin XVI, Nex-1)

Ayano Satoh, M. Hazuki, K. Kojima, J. Hirabayashi, I. Matsumoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Annexins are structurally related proteins that bind phospholipids in a calcium-dependent manner. Recently, we showed that annexins IV, V, and VI also bind glycosaminoglycans in a calcium-dependent manner. Annexins are widely distributed from lower to higher eukaryotes, and the nematode Caenorhabditis elegans has been found to contain Nex-1, an annexin homologue. Here, we characterize the ligand-binding properties of Nex-1 using recombinant Nex-1. Nex-1 binds to liposomes containing phosphatidylserine. The apparent K(d) was calculated by Biacore to be 4.4 nM. Compared to mammalian annexins, the Nex-1 phospholipid-binding specificities were similar whereas the K(d) values were one order of magnitude larger. The Nex-1 glycosaminoglycan-binding specificities were investigated by affinity chromatography and solid-phase assays. Nex-1 binds to heparin, heparan sulfate, and chondroitin sulfate but not to chondroitin and chemically N- or O-desulfated heparin. Besides phospholipids, heparan sulfate and/or chondroitin (sulfate), probably on perlecan, could be endogenous ligands of Nex-1.

Original languageEnglish
Pages (from-to)377-381
Number of pages5
JournalJournal of Biochemistry
Volume128
Issue number3
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Annexins
Caenorhabditis elegans
Ligands
Phospholipids
Heparitin Sulfate
Chondroitin Sulfates
Glycosaminoglycans
Annexin A4
Annexin A6
Chondroitin
Calcium
Affinity chromatography
Annexin A5
Phosphatidylserines
Eukaryota
Affinity Chromatography
Liposomes
Heparin
Assays
Proteins

Keywords

  • Annexin
  • Glycosaminoglycan
  • Phospholipid

ASJC Scopus subject areas

  • Biochemistry

Cite this

Satoh, A., Hazuki, M., Kojima, K., Hirabayashi, J., & Matsumoto, I. (2000). Ligand-binding properties of annexin from Caenorhabditis elegans (Annexin XVI, Nex-1). Journal of Biochemistry, 128(3), 377-381.

Ligand-binding properties of annexin from Caenorhabditis elegans (Annexin XVI, Nex-1). / Satoh, Ayano; Hazuki, M.; Kojima, K.; Hirabayashi, J.; Matsumoto, I.

In: Journal of Biochemistry, Vol. 128, No. 3, 2000, p. 377-381.

Research output: Contribution to journalArticle

Satoh, A, Hazuki, M, Kojima, K, Hirabayashi, J & Matsumoto, I 2000, 'Ligand-binding properties of annexin from Caenorhabditis elegans (Annexin XVI, Nex-1)', Journal of Biochemistry, vol. 128, no. 3, pp. 377-381.
Satoh, Ayano ; Hazuki, M. ; Kojima, K. ; Hirabayashi, J. ; Matsumoto, I. / Ligand-binding properties of annexin from Caenorhabditis elegans (Annexin XVI, Nex-1). In: Journal of Biochemistry. 2000 ; Vol. 128, No. 3. pp. 377-381.
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