TY - JOUR
T1 - Lidocaine protects hippocampal neurons against ischemic damage by preventing increase of extracellular excitatory amino acids
T2 - a microdialysis study in Mongolian gerbils
AU - Fujitani, Taro
AU - Adachi, Naoto
AU - Miyazaki, Hirofumi
AU - Liu, Keyue
AU - Nakamura, Yoichi
AU - Kataoka, Kiyoshi
AU - Arai, Tatsuru
PY - 1994/9/26
Y1 - 1994/9/26
N2 - Extracellular levels of amino acids in the hippocampal CA1 region of the gerbil were analyzed by a microdialysis-HPLC procedure. Transient forebrain ischemia produced significant increase in aspartate, glutamate, glycine and taurine (760%, 1070%, 190% and 1210%, respectively), and neuronal blockade by perfusion with a lidocaine (4 mM)-containing medium resulted in 67%, 79%, 58% and 59% reduction in the peak values of each amino acid, respectively. On the other hand, an intracerebroventricular administration of lidocaine, 0.8 μmol or more, produced a protective effect against delayed damage of hippocampal CA1 pyramidal cells, which was caused by bilateral carotid artery occlusion for 4 min. The results suggest that lidocaine may protect neurons against ischemic damage, by preventing the ischemia-induced rise of extracellular concentration of excitatory amino acid.
AB - Extracellular levels of amino acids in the hippocampal CA1 region of the gerbil were analyzed by a microdialysis-HPLC procedure. Transient forebrain ischemia produced significant increase in aspartate, glutamate, glycine and taurine (760%, 1070%, 190% and 1210%, respectively), and neuronal blockade by perfusion with a lidocaine (4 mM)-containing medium resulted in 67%, 79%, 58% and 59% reduction in the peak values of each amino acid, respectively. On the other hand, an intracerebroventricular administration of lidocaine, 0.8 μmol or more, produced a protective effect against delayed damage of hippocampal CA1 pyramidal cells, which was caused by bilateral carotid artery occlusion for 4 min. The results suggest that lidocaine may protect neurons against ischemic damage, by preventing the ischemia-induced rise of extracellular concentration of excitatory amino acid.
KW - Amino acids
KW - CA1
KW - Cerebral ischemia
KW - Hippocampus
KW - Lidocaine
KW - Microdialysis
KW - Mongolian gerbils
UR - http://www.scopus.com/inward/record.url?scp=0027966070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027966070&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(94)90942-3
DO - 10.1016/0304-3940(94)90942-3
M3 - Article
C2 - 7845632
AN - SCOPUS:0027966070
VL - 179
SP - 91
EP - 94
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1-2
ER -