Levels of sister-chromatid exchanges in hybrids between Bloom syndrome B-lymphoblastoid cells and various cell lines with lymphoid malignancy

Yukimasa Shiraishi, Hirotsugu Kobuchi, Kozo Utsumi, Jun Minowada

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Abstract

The present study has been undertaken to examine the effect of cell hybridization of Bloom syndrome (BS) B-lymphoblastoid cell lines (LCLs) and various cell lines from lymphoid malignancies in order to clarify the relationship between sister-chromatid exchange (SCE) and malignant conditions. Cell hybridization studies have shown that though BS high-SCE frequencies were completed by fusion with normal cells, fusion with various malignant cell lines did not result in complete normalization of BS SCEs, with 15-30 SCEs remaining per hybrid cell, demonstrating possibly common defects in DNA of BS and malignant cells. These findings strongly support the idea that the characteristic high SCE frequency in BS cells has some connection with the malignant condition, and that at least one step in carcinogenesis is either accompanied by the production of SCEs, or that SCEs themselves cause such a step to occur.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalMutation Research Letters
Volume243
Issue number1
DOIs
Publication statusPublished - 1990
Externally publishedYes

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Keywords

  • Bloom syndrome cells
  • Cell hybridization
  • Endoreduplication
  • Lymphoid malignant cell lines
  • SCE level

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

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