TY - JOUR
T1 - Levels of sister-chromatid exchanges in hybrids between Bloom syndrome B-lymphoblastoid cells and various cell lines with lymphoid malignancy
AU - Shiraishi, Yukimasa
AU - Kobuchi, Hirotsugu
AU - Utsumi, Kozo
AU - Minowada, Jun
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan. The authors are grateful to Miss Michiyo Ozawa for her technical assistance and photographic services.
PY - 1990/1
Y1 - 1990/1
N2 - The present study has been undertaken to examine the effect of cell hybridization of Bloom syndrome (BS) B-lymphoblastoid cell lines (LCLs) and various cell lines from lymphoid malignancies in order to clarify the relationship between sister-chromatid exchange (SCE) and malignant conditions. Cell hybridization studies have shown that though BS high-SCE frequencies were completed by fusion with normal cells, fusion with various malignant cell lines did not result in complete normalization of BS SCEs, with 15-30 SCEs remaining per hybrid cell, demonstrating possibly common defects in DNA of BS and malignant cells. These findings strongly support the idea that the characteristic high SCE frequency in BS cells has some connection with the malignant condition, and that at least one step in carcinogenesis is either accompanied by the production of SCEs, or that SCEs themselves cause such a step to occur.
AB - The present study has been undertaken to examine the effect of cell hybridization of Bloom syndrome (BS) B-lymphoblastoid cell lines (LCLs) and various cell lines from lymphoid malignancies in order to clarify the relationship between sister-chromatid exchange (SCE) and malignant conditions. Cell hybridization studies have shown that though BS high-SCE frequencies were completed by fusion with normal cells, fusion with various malignant cell lines did not result in complete normalization of BS SCEs, with 15-30 SCEs remaining per hybrid cell, demonstrating possibly common defects in DNA of BS and malignant cells. These findings strongly support the idea that the characteristic high SCE frequency in BS cells has some connection with the malignant condition, and that at least one step in carcinogenesis is either accompanied by the production of SCEs, or that SCEs themselves cause such a step to occur.
KW - Bloom syndrome cells
KW - Cell hybridization
KW - Endoreduplication
KW - Lymphoid malignant cell lines
KW - SCE level
UR - http://www.scopus.com/inward/record.url?scp=0025190625&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025190625&partnerID=8YFLogxK
U2 - 10.1016/0165-7992(90)90117-3
DO - 10.1016/0165-7992(90)90117-3
M3 - Article
C2 - 2300080
AN - SCOPUS:0025190625
SN - 0165-7992
VL - 243
SP - 13
EP - 20
JO - Mutation Research Letters
JF - Mutation Research Letters
IS - 1
ER -