Levels of human defensin-1, an antimicrobial peptide, in saliva of patients with oral inflammation

Nobuyoshi Mizukawa, Katsumi Sugiyama, Takaaki Uenokatsuaki Mishima, Shin Takagi, Toshio Sugahara

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Objective. The purpose of this study was to investigate the presence of an antimicrobial peptide, human defensin-1, in the saliva of patients with oral inflammation. Study design. Whole saliva samples were collected from patients with oral inflammation and from healthy volunteers. Human defensin-1 in saliva was isolated and purified by reversed-phase high-performance liquid chromatography. The amino acid sequence and molecular weight of defensin-1 were determined. The concentration of defensin-1 in saliva was quantified by high-performance liquid chromatography. Serum C-reactive protein concentration was measured by particle-enhanced turbidi-metric immunoassay. Results. The salivary defensin-1 concentration was significantly higher in patients with oral inflammation than in healthy volunteers; furthermore, in patients with oral inflammation, the concentration was significantly higher before treatment than after treatment. In the patients with oral inflammation, there was a strong positive correlation between salivary defensin-1 concentration and serum C-reactive protein concentration. Conclusions. The findings suggest that defensin-1 in saliva may be a convenient marker of inflammation associated with oral disease.

Original languageEnglish
Pages (from-to)539-543
Number of pages5
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume87
Issue number5
Publication statusPublished - 1999

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Defensins
Saliva
Inflammation
Peptides
C-Reactive Protein
Blood Proteins
Healthy Volunteers
Mouth Diseases
High Pressure Liquid Chromatography
Reverse-Phase Chromatography
Immunoassay
Amino Acid Sequence
Molecular Weight
Therapeutics

ASJC Scopus subject areas

  • Dentistry(all)
  • Pathology and Forensic Medicine
  • Radiology Nuclear Medicine and imaging
  • Surgery

Cite this

Levels of human defensin-1, an antimicrobial peptide, in saliva of patients with oral inflammation. / Mizukawa, Nobuyoshi; Sugiyama, Katsumi; Mishima, Takaaki Uenokatsuaki; Takagi, Shin; Sugahara, Toshio.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol. 87, No. 5, 1999, p. 539-543.

Research output: Contribution to journalArticle

Mizukawa, Nobuyoshi ; Sugiyama, Katsumi ; Mishima, Takaaki Uenokatsuaki ; Takagi, Shin ; Sugahara, Toshio. / Levels of human defensin-1, an antimicrobial peptide, in saliva of patients with oral inflammation. In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. 1999 ; Vol. 87, No. 5. pp. 539-543.
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