Leukotrienes affect secretory function of ovarian cells in vitro

A. J. Korzekwa, T. J. Acosta, M. Miklewicz, K. Okuda, S. H. Lee, D. J. Skarzynski

Research output: Contribution to journalArticle

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Abstract

Contents: The aim of this study was to determine which cells are the source of production and target for leukotriene (LTs) action within the bovine ovary. Luteal (CL, days 14-16 of the oestrous cycle), steroidogenic cells (LSC) and endothelial cells (LEC) of the bovine corpus luteum (CL), and granulosa cells (GC) were isolated enzymatically, cultured in a monolayer and incubated with LTC4, LTB4, Azelastine (an antagonist of LTC4) or Dapsone (an antagonist of LTB4). Then cells were collected for determination of mRNA expression for LT receptors (LTRs) and 5-lipoxygenase (5-LO) by real time RT-PCR, and media were collected for determination of prostaglandin (PG)E2, F, progesterone (P4; LSC only), endothelin-1 (ET-1; LEC only) and 17-β oestradiol (E2; GC only). The greatest mRNA expression for LTR-II and 5-LO were found in LEC, whereas LTR-I mRNA expression did not differ among cell types. The level of PGE2 increased after LTs treatment in each type of ovarian cell, excluding LTC4 treatment in LEC. The secretion of PGF was also increased by LTs, but decreased after LTB4 treatment of LSC. In GC cultures, both LTs stimulated E2 secretion; in LEC cultures, LTB4 stimulated whereas LTC4 inhibited P4 secretion; in LEC cultures, LTC4 stimulated but LTB4 inhibited ET-1 secretion. The results show that LTs are produced locally and are involved in PGs production/secretion in all examined cells (LSC, LEC and GC) of bovine ovary. Leukotriene treatment modulate secretion of E2, by GC, P4 by LSC and ET-1 by LEC, which indicates that LTs are involved in regulation of ovarian secretory functions.

Original languageEnglish
JournalReproduction in Domestic Animals
Volume45
Issue number6
DOIs
Publication statusPublished - Dec 2010

Fingerprint

leukotrienes
Leukotrienes
endothelial cells
Leukotriene C4
Endothelial Cells
Leukotriene B4
Granulosa Cells
granulosa cells
secretion
Corpus Luteum
corpus luteum
cells
Arachidonate 5-Lipoxygenase
cell culture
Dinoprost
Cell Culture Techniques
azelastine
lipoxygenase
Dinoprostone
Messenger RNA

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Endocrinology

Cite this

Korzekwa, A. J., Acosta, T. J., Miklewicz, M., Okuda, K., Lee, S. H., & Skarzynski, D. J. (2010). Leukotrienes affect secretory function of ovarian cells in vitro. Reproduction in Domestic Animals, 45(6). https://doi.org/10.1111/j.1439-0531.2009.01559.x

Leukotrienes affect secretory function of ovarian cells in vitro. / Korzekwa, A. J.; Acosta, T. J.; Miklewicz, M.; Okuda, K.; Lee, S. H.; Skarzynski, D. J.

In: Reproduction in Domestic Animals, Vol. 45, No. 6, 12.2010.

Research output: Contribution to journalArticle

Korzekwa, AJ, Acosta, TJ, Miklewicz, M, Okuda, K, Lee, SH & Skarzynski, DJ 2010, 'Leukotrienes affect secretory function of ovarian cells in vitro', Reproduction in Domestic Animals, vol. 45, no. 6. https://doi.org/10.1111/j.1439-0531.2009.01559.x
Korzekwa, A. J. ; Acosta, T. J. ; Miklewicz, M. ; Okuda, K. ; Lee, S. H. ; Skarzynski, D. J. / Leukotrienes affect secretory function of ovarian cells in vitro. In: Reproduction in Domestic Animals. 2010 ; Vol. 45, No. 6.
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