Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness

Nobuaki Miyahara, Katsuyuki Takeda, Satoko Miyahara, Christian Taube, Anthony Joetham, Toshiyuki Koya, Shigeki Matsubara, Azzeddine Dakhama, Andrew M. Tager, Andrew D. Luster, Erwin W. Gelfand

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Recent studies in both human and rodents have indicated that in addition to CD4+ T cells, CD8+ T cells play an important role in allergic inflammation. We previously demonstrated that allergen-sensitized and -challenged CD8-deficient (CD8-/-) mice develop significantly lower airway hyperresponsiveness (AHR), eosinophilic inflammation, and IL-13 levels in bronchoalveolar lavage fluid compared with wild-type mice, and that all these responses were restored by adoptive transfer of in vivo-primed CD8+ T cells or in vitro-generated effector CD8+ T cells (TEFF). Recently, leukotriene B4 and its high affinity receptor, BLT1, have been shown to mediate in vitro-generated TEFF recruitment into inflamed tissues. In this study we investigated whether BLT1 is essential for the development of CD8+ T cell-mediated allergic AHR and inflammation. Adoptive transfer of in vivo-primed BLT1+/+, but not BLT1-/-, CD8 + T cells into sensitized and challenged CD8-/- mice restored AHR, eosinophilic inflammation, and IL-13 levels. Moreover, when adoptively transferred into sensitized CD8-/- mice, in vitro-generated BLT1+/+, but not BLT1-/-, TEFF accumulated in the lung and mediated these altered airway responses to allergen challenge. These data are the first to show both a functional and an essential role for BLT1 in allergen-mediated CD8+ TEFF recruitment into the lung and development of AHR and airway inflammation.

Original languageEnglish
Pages (from-to)4979-4984
Number of pages6
JournalJournal of Immunology
Volume174
Issue number8
Publication statusPublished - Apr 15 2005
Externally publishedYes

Fingerprint

Leukotriene B4 Receptors
Allergens
T-Lymphocytes
Inflammation
Interleukin-13
Adoptive Transfer
Lung
Leukotriene B4
Bronchoalveolar Lavage Fluid
Rodentia

ASJC Scopus subject areas

  • Immunology

Cite this

Miyahara, N., Takeda, K., Miyahara, S., Taube, C., Joetham, A., Koya, T., ... Gelfand, E. W. (2005). Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness. Journal of Immunology, 174(8), 4979-4984.

Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness. / Miyahara, Nobuaki; Takeda, Katsuyuki; Miyahara, Satoko; Taube, Christian; Joetham, Anthony; Koya, Toshiyuki; Matsubara, Shigeki; Dakhama, Azzeddine; Tager, Andrew M.; Luster, Andrew D.; Gelfand, Erwin W.

In: Journal of Immunology, Vol. 174, No. 8, 15.04.2005, p. 4979-4984.

Research output: Contribution to journalArticle

Miyahara, N, Takeda, K, Miyahara, S, Taube, C, Joetham, A, Koya, T, Matsubara, S, Dakhama, A, Tager, AM, Luster, AD & Gelfand, EW 2005, 'Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness', Journal of Immunology, vol. 174, no. 8, pp. 4979-4984.
Miyahara, Nobuaki ; Takeda, Katsuyuki ; Miyahara, Satoko ; Taube, Christian ; Joetham, Anthony ; Koya, Toshiyuki ; Matsubara, Shigeki ; Dakhama, Azzeddine ; Tager, Andrew M. ; Luster, Andrew D. ; Gelfand, Erwin W. / Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness. In: Journal of Immunology. 2005 ; Vol. 174, No. 8. pp. 4979-4984.
@article{c7e711a4a3cc4b119c44fdc76d0a840f,
title = "Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness",
abstract = "Recent studies in both human and rodents have indicated that in addition to CD4+ T cells, CD8+ T cells play an important role in allergic inflammation. We previously demonstrated that allergen-sensitized and -challenged CD8-deficient (CD8-/-) mice develop significantly lower airway hyperresponsiveness (AHR), eosinophilic inflammation, and IL-13 levels in bronchoalveolar lavage fluid compared with wild-type mice, and that all these responses were restored by adoptive transfer of in vivo-primed CD8+ T cells or in vitro-generated effector CD8+ T cells (TEFF). Recently, leukotriene B4 and its high affinity receptor, BLT1, have been shown to mediate in vitro-generated TEFF recruitment into inflamed tissues. In this study we investigated whether BLT1 is essential for the development of CD8+ T cell-mediated allergic AHR and inflammation. Adoptive transfer of in vivo-primed BLT1+/+, but not BLT1-/-, CD8 + T cells into sensitized and challenged CD8-/- mice restored AHR, eosinophilic inflammation, and IL-13 levels. Moreover, when adoptively transferred into sensitized CD8-/- mice, in vitro-generated BLT1+/+, but not BLT1-/-, TEFF accumulated in the lung and mediated these altered airway responses to allergen challenge. These data are the first to show both a functional and an essential role for BLT1 in allergen-mediated CD8+ TEFF recruitment into the lung and development of AHR and airway inflammation.",
author = "Nobuaki Miyahara and Katsuyuki Takeda and Satoko Miyahara and Christian Taube and Anthony Joetham and Toshiyuki Koya and Shigeki Matsubara and Azzeddine Dakhama and Tager, {Andrew M.} and Luster, {Andrew D.} and Gelfand, {Erwin W.}",
year = "2005",
month = "4",
day = "15",
language = "English",
volume = "174",
pages = "4979--4984",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness

AU - Miyahara, Nobuaki

AU - Takeda, Katsuyuki

AU - Miyahara, Satoko

AU - Taube, Christian

AU - Joetham, Anthony

AU - Koya, Toshiyuki

AU - Matsubara, Shigeki

AU - Dakhama, Azzeddine

AU - Tager, Andrew M.

AU - Luster, Andrew D.

AU - Gelfand, Erwin W.

PY - 2005/4/15

Y1 - 2005/4/15

N2 - Recent studies in both human and rodents have indicated that in addition to CD4+ T cells, CD8+ T cells play an important role in allergic inflammation. We previously demonstrated that allergen-sensitized and -challenged CD8-deficient (CD8-/-) mice develop significantly lower airway hyperresponsiveness (AHR), eosinophilic inflammation, and IL-13 levels in bronchoalveolar lavage fluid compared with wild-type mice, and that all these responses were restored by adoptive transfer of in vivo-primed CD8+ T cells or in vitro-generated effector CD8+ T cells (TEFF). Recently, leukotriene B4 and its high affinity receptor, BLT1, have been shown to mediate in vitro-generated TEFF recruitment into inflamed tissues. In this study we investigated whether BLT1 is essential for the development of CD8+ T cell-mediated allergic AHR and inflammation. Adoptive transfer of in vivo-primed BLT1+/+, but not BLT1-/-, CD8 + T cells into sensitized and challenged CD8-/- mice restored AHR, eosinophilic inflammation, and IL-13 levels. Moreover, when adoptively transferred into sensitized CD8-/- mice, in vitro-generated BLT1+/+, but not BLT1-/-, TEFF accumulated in the lung and mediated these altered airway responses to allergen challenge. These data are the first to show both a functional and an essential role for BLT1 in allergen-mediated CD8+ TEFF recruitment into the lung and development of AHR and airway inflammation.

AB - Recent studies in both human and rodents have indicated that in addition to CD4+ T cells, CD8+ T cells play an important role in allergic inflammation. We previously demonstrated that allergen-sensitized and -challenged CD8-deficient (CD8-/-) mice develop significantly lower airway hyperresponsiveness (AHR), eosinophilic inflammation, and IL-13 levels in bronchoalveolar lavage fluid compared with wild-type mice, and that all these responses were restored by adoptive transfer of in vivo-primed CD8+ T cells or in vitro-generated effector CD8+ T cells (TEFF). Recently, leukotriene B4 and its high affinity receptor, BLT1, have been shown to mediate in vitro-generated TEFF recruitment into inflamed tissues. In this study we investigated whether BLT1 is essential for the development of CD8+ T cell-mediated allergic AHR and inflammation. Adoptive transfer of in vivo-primed BLT1+/+, but not BLT1-/-, CD8 + T cells into sensitized and challenged CD8-/- mice restored AHR, eosinophilic inflammation, and IL-13 levels. Moreover, when adoptively transferred into sensitized CD8-/- mice, in vitro-generated BLT1+/+, but not BLT1-/-, TEFF accumulated in the lung and mediated these altered airway responses to allergen challenge. These data are the first to show both a functional and an essential role for BLT1 in allergen-mediated CD8+ TEFF recruitment into the lung and development of AHR and airway inflammation.

UR - http://www.scopus.com/inward/record.url?scp=20144389172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144389172&partnerID=8YFLogxK

M3 - Article

C2 - 15814727

AN - SCOPUS:20144389172

VL - 174

SP - 4979

EP - 4984

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -