Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8+ T cells and airway hyperresponsiveness

Nobuaki Miyahara, Katsuyuki Takeda, Satoko Miyahara, Christian Taube, Anthony Joetham, Toshiyuki Koya, Shigeki Matsubara, Azzeddine Dakhama, Andrew M. Tager, Andrew D. Luster, Erwin W. Gelfand

Research output: Contribution to journalArticlepeer-review

97 Citations (Scopus)

Abstract

Recent studies in both human and rodents have indicated that in addition to CD4+ T cells, CD8+ T cells play an important role in allergic inflammation. We previously demonstrated that allergen-sensitized and -challenged CD8-deficient (CD8-/-) mice develop significantly lower airway hyperresponsiveness (AHR), eosinophilic inflammation, and IL-13 levels in bronchoalveolar lavage fluid compared with wild-type mice, and that all these responses were restored by adoptive transfer of in vivo-primed CD8+ T cells or in vitro-generated effector CD8+ T cells (TEFF). Recently, leukotriene B4 and its high affinity receptor, BLT1, have been shown to mediate in vitro-generated TEFF recruitment into inflamed tissues. In this study we investigated whether BLT1 is essential for the development of CD8+ T cell-mediated allergic AHR and inflammation. Adoptive transfer of in vivo-primed BLT1+/+, but not BLT1-/-, CD8 + T cells into sensitized and challenged CD8-/- mice restored AHR, eosinophilic inflammation, and IL-13 levels. Moreover, when adoptively transferred into sensitized CD8-/- mice, in vitro-generated BLT1+/+, but not BLT1-/-, TEFF accumulated in the lung and mediated these altered airway responses to allergen challenge. These data are the first to show both a functional and an essential role for BLT1 in allergen-mediated CD8+ TEFF recruitment into the lung and development of AHR and airway inflammation.

Original languageEnglish
Pages (from-to)4979-4984
Number of pages6
JournalJournal of Immunology
Volume174
Issue number8
DOIs
Publication statusPublished - Apr 15 2005
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Leukotriene B4 receptor-1 is essential for allergen-mediated recruitment of CD8<sup>+</sup> T cells and airway hyperresponsiveness'. Together they form a unique fingerprint.

Cite this