Leukemia-associated fusion proteins, dek-can and bcr-abl, represent immunogenic HLA-DR-restricted epitopes recognized by fusion peptide-specific CD4+ T lymphocytes

M. Makita, T. Azuma, H. Hamaguchi, H. Niiya, K. Kojima, S. Fujita, M. Tanimoto, M. Harada, M. Yasukawa

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Although CD4+ helper T lymphocytes have been demonstrated to play an important role in antitumor immune response, only a few epitopes of tumor-associated antigens recognized by HLA class II-restricted CD4+ T lymphocytes have been identified. In the present study, we addressed the question of whether leukemia-associated fusion proteins are recognized by CD4+ T lymphocytes. Immature dendritic cells (DCs) were loaded with necrotic or apoptotic leukemia cells with t(6;9) or t(9;22) and then cocultured with the dek-can fusion peptide-specific or the bcr-abl fusion peptide-specific CD4+ T lymphocyte clone. The dek-can peptide-specific and bcr-abl peptide-specific CD4+ T lymphocyte clones produced interferon-γ (IFN-γ) when they were cocultured with HLA-DR-matched but not with mismatched DCs which had been loaded with apoptotic as well as necrotic leukemia cells with t(6;9) and t(9;22), respectively. IFN-γ production by CN4+T lymphocyte clones in response to stimulation with DCs loaded with leukemia cells was inhibited by the anti-HLA-DR monoclonal antibody. These data indicate that the acute myelogenous leukemia-associated fusion protein, dek-can, and chronic myelogenous leukemia-associated fusion protein, bcr-abl, are both processed and presented by DCs to the fusion peptide-specific CD4+ T lymphocytes.

Original languageEnglish
Pages (from-to)2400-2407
Number of pages8
JournalLeukemia
Volume16
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

HLA-DR Antigens
Epitopes
Leukemia
T-Lymphocytes
Dendritic Cells
Peptides
Proteins
Clone Cells
Interferons
bcr-abl Fusion Proteins
Cell Fusion
Neoplasm Antigens
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Helper-Inducer T-Lymphocytes
Acute Myeloid Leukemia
Monoclonal Antibodies

Keywords

  • CD T lymphocytes
  • Dendritic cells
  • Immunotherapy
  • Leukemia
  • Tumor-associated antigens

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Leukemia-associated fusion proteins, dek-can and bcr-abl, represent immunogenic HLA-DR-restricted epitopes recognized by fusion peptide-specific CD4+ T lymphocytes. / Makita, M.; Azuma, T.; Hamaguchi, H.; Niiya, H.; Kojima, K.; Fujita, S.; Tanimoto, M.; Harada, M.; Yasukawa, M.

In: Leukemia, Vol. 16, No. 12, 01.12.2002, p. 2400-2407.

Research output: Contribution to journalArticle

Makita, M, Azuma, T, Hamaguchi, H, Niiya, H, Kojima, K, Fujita, S, Tanimoto, M, Harada, M & Yasukawa, M 2002, 'Leukemia-associated fusion proteins, dek-can and bcr-abl, represent immunogenic HLA-DR-restricted epitopes recognized by fusion peptide-specific CD4+ T lymphocytes', Leukemia, vol. 16, no. 12, pp. 2400-2407. https://doi.org/10.1038/sj.leu.2402742
Makita, M. ; Azuma, T. ; Hamaguchi, H. ; Niiya, H. ; Kojima, K. ; Fujita, S. ; Tanimoto, M. ; Harada, M. ; Yasukawa, M. / Leukemia-associated fusion proteins, dek-can and bcr-abl, represent immunogenic HLA-DR-restricted epitopes recognized by fusion peptide-specific CD4+ T lymphocytes. In: Leukemia. 2002 ; Vol. 16, No. 12. pp. 2400-2407.
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