Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids

Masumi Taki, Atsushi Kuno, Shinsuke Matoba, Yuki Kobayashi, Junichiro Futami, Hiroshi Murakami, Hiroaki Suga, Kazunari Taira, Tsunemi Hasegawa, Masahiko Sisido

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

Original languageEnglish
Pages (from-to)1676-1679
Number of pages4
JournalChemBioChem
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 2006

Fingerprint

leucyltransferase
Amino Acids
Transfer RNA
Proteins
Transferases

Keywords

  • Amino acids
  • N-terminal-specific labeling
  • Peptides
  • Protein modifications
  • tRNA

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. / Taki, Masumi; Kuno, Atsushi; Matoba, Shinsuke; Kobayashi, Yuki; Futami, Junichiro; Murakami, Hiroshi; Suga, Hiroaki; Taira, Kazunari; Hasegawa, Tsunemi; Sisido, Masahiko.

In: ChemBioChem, Vol. 7, No. 11, 11.2006, p. 1676-1679.

Research output: Contribution to journalArticle

Taki, Masumi ; Kuno, Atsushi ; Matoba, Shinsuke ; Kobayashi, Yuki ; Futami, Junichiro ; Murakami, Hiroshi ; Suga, Hiroaki ; Taira, Kazunari ; Hasegawa, Tsunemi ; Sisido, Masahiko. / Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. In: ChemBioChem. 2006 ; Vol. 7, No. 11. pp. 1676-1679.
@article{8346f4a50a094564abd9aff0dd8ffa32,
title = "Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids",
abstract = "(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.",
keywords = "Amino acids, N-terminal-specific labeling, Peptides, Protein modifications, tRNA",
author = "Masumi Taki and Atsushi Kuno and Shinsuke Matoba and Yuki Kobayashi and Junichiro Futami and Hiroshi Murakami and Hiroaki Suga and Kazunari Taira and Tsunemi Hasegawa and Masahiko Sisido",
year = "2006",
month = "11",
doi = "10.1002/cbic.200600181",
language = "English",
volume = "7",
pages = "1676--1679",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "11",

}

TY - JOUR

T1 - Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids

AU - Taki, Masumi

AU - Kuno, Atsushi

AU - Matoba, Shinsuke

AU - Kobayashi, Yuki

AU - Futami, Junichiro

AU - Murakami, Hiroshi

AU - Suga, Hiroaki

AU - Taira, Kazunari

AU - Hasegawa, Tsunemi

AU - Sisido, Masahiko

PY - 2006/11

Y1 - 2006/11

N2 - (Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

AB - (Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

KW - Amino acids

KW - N-terminal-specific labeling

KW - Peptides

KW - Protein modifications

KW - tRNA

UR - http://www.scopus.com/inward/record.url?scp=33750997879&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750997879&partnerID=8YFLogxK

U2 - 10.1002/cbic.200600181

DO - 10.1002/cbic.200600181

M3 - Article

C2 - 16977663

AN - SCOPUS:33750997879

VL - 7

SP - 1676

EP - 1679

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 11

ER -