Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids

Masumi Taki; Atsushi Kuno; Shinsuke Matoba; Yuki Kobayashi; Junichiro Futami; Hiroshi Murakami; Hiroaki Suga; Kazunari Taira; Tsunemi Hasegawa; Masahiko Sisido

doi:10.1002/cbic.200600181

Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids

Masumi Taki, Atsushi Kuno, Shinsuke Matoba, Yuki Kobayashi, Junichiro Futami, Hiroshi Murakami, Hiroaki Suga, Kazunari Taira, Tsunemi Hasegawa, Masahiko Sisido

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

Original language	English
Pages (from-to)	1676-1679
Number of pages	4
Journal	ChemBioChem
Volume	7
Issue number	11
DOIs	https://doi.org/10.1002/cbic.200600181
Publication status	Published - Nov 2006

Keywords

Amino acids
N-terminal-specific labeling
Peptides
Protein modifications
tRNA

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200600181

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Taki, M., Kuno, A., Matoba, S., Kobayashi, Y., Futami, J., Murakami, H., Suga, H., Taira, K., Hasegawa, T., & Sisido, M. (2006). Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. ChemBioChem, 7(11), 1676-1679. https://doi.org/10.1002/cbic.200600181

Taki, M, Kuno, A, Matoba, S, Kobayashi, Y, Futami, J, Murakami, H, Suga, H, Taira, K, Hasegawa, T & Sisido, M 2006, 'Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids', ChemBioChem, vol. 7, no. 11, pp. 1676-1679. https://doi.org/10.1002/cbic.200600181

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abstract = "(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.",

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author = "Masumi Taki and Atsushi Kuno and Shinsuke Matoba and Yuki Kobayashi and Junichiro Futami and Hiroshi Murakami and Hiroaki Suga and Kazunari Taira and Tsunemi Hasegawa and Masahiko Sisido",

year = "2006",

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doi = "10.1002/cbic.200600181",

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AU - Taki, Masumi

AU - Kuno, Atsushi

AU - Matoba, Shinsuke

AU - Kobayashi, Yuki

AU - Futami, Junichiro

AU - Murakami, Hiroshi

AU - Suga, Hiroaki

AU - Taira, Kazunari

AU - Hasegawa, Tsunemi

AU - Sisido, Masahiko

PY - 2006/11

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AB - (Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

KW - Amino acids

KW - N-terminal-specific labeling

KW - Peptides

KW - Protein modifications

KW - tRNA

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Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids

Abstract

Keywords

ASJC Scopus subject areas

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