Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids

Masumi Taki, Atsushi Kuno, Shinsuke Matoba, Yuki Kobayashi, Junichiro Futami, Hiroshi Murakami, Hiroaki Suga, Kazunari Taira, Tsunemi Hasegawa, Masahiko Sisido

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

Original languageEnglish
Pages (from-to)1676-1679
Number of pages4
JournalChemBioChem
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 1 2006

Keywords

  • Amino acids
  • N-terminal-specific labeling
  • Peptides
  • Protein modifications
  • tRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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