Abstract
(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.
Original language | English |
---|---|
Pages (from-to) | 1676-1679 |
Number of pages | 4 |
Journal | ChemBioChem |
Volume | 7 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2006 |
Keywords
- Amino acids
- N-terminal-specific labeling
- Peptides
- Protein modifications
- tRNA
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Organic Chemistry