Abstract
(Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.
| Original language | English |
|---|---|
| Pages (from-to) | 1676-1679 |
| Number of pages | 4 |
| Journal | ChemBioChem |
| Volume | 7 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2006 |
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Keywords
- Amino acids
- N-terminal-specific labeling
- Peptides
- Protein modifications
- tRNA
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. / Taki, Masumi; Kuno, Atsushi; Matoba, Shinsuke; Kobayashi, Yuki; Futami, Junichiro; Murakami, Hiroshi; Suga, Hiroaki; Taira, Kazunari; Hasegawa, Tsunemi; Sisido, Masahiko.
In: ChemBioChem, Vol. 7, No. 11, 11.2006, p. 1676-1679.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Leucyl/phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids
AU - Taki, Masumi
AU - Kuno, Atsushi
AU - Matoba, Shinsuke
AU - Kobayashi, Yuki
AU - Futami, Junichiro
AU - Murakami, Hiroshi
AU - Suga, Hiroaki
AU - Taira, Kazunari
AU - Hasegawa, Tsunemi
AU - Sisido, Masahiko
PY - 2006/11
Y1 - 2006/11
N2 - (Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.
AB - (Chemical Equation Presented) The protein no longer terminates here. Non-natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F-tRNA-protein transferase to the N termini of proteins possessing N-terminal Lys or Arg components. By this new technique, 1- and 2-naphthylalanine or 3-nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.
KW - Amino acids
KW - N-terminal-specific labeling
KW - Peptides
KW - Protein modifications
KW - tRNA
UR - http://www.scopus.com/inward/record.url?scp=33750997879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750997879&partnerID=8YFLogxK
U2 - 10.1002/cbic.200600181
DO - 10.1002/cbic.200600181
M3 - Article
C2 - 16977663
AN - SCOPUS:33750997879
VL - 7
SP - 1676
EP - 1679
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 11
ER -