Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1

Mari Narusaka, Kazuhiro Toyoda, Tomonori Shiraishi, Satoshi Iuchi, Yoshitaka Takano, Ken Shirasu, Yoshihiro Narusaka

Research output: Contribution to journalArticle

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Abstract

Arabidopsis thaliana leucine-rich repeat-containing (NLR) proteins RPS4 and RRS1, known as dual resistance proteins, confer resistance to multiple pathogen isolates, such as the bacterial pathogens Pseudomonas syringae and Ralstonia solanacearum and the fungal pathogen Colletotrichum higginsianum. RPS4 is a typical Toll/interleukin 1 Receptor (TIR)-type NLR, whereas RRS1 is an atypical TIR-NLR that contains a leucine zipper (LZ) motif and a C-terminal WRKY domain. RPS4 and RRS1 are localised near each other in a head-to-head orientation. In this study, direct mutagenesis of the C-terminal LZ motif in RRS1 caused an autoimmune response and stunting in the mutant. Co-immunoprecipitation analysis indicated that full-length RPS4 and RRS1 are physically associated with one another. Furthermore, virus-induced gene silencing experiments showed that hypersensitive-like cell death triggered by RPS4/LZ motif-mutated RRS1 depends on EDS1. In conclusion, we suggest that the RRS1-LZ motif is crucial for the regulation of the RPS4/RRS1 complex.

Original languageEnglish
Article number18702
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Jan 11 2016

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Leucine Zippers
Arabidopsis
Ralstonia solanacearum
Interleukin-1 Receptors
Proteins
Growth Disorders
Colletotrichum
Pseudomonas syringae
Gene Silencing
Autoimmunity
Immunoprecipitation
Leucine
Mutagenesis
Cell Death
Viruses

ASJC Scopus subject areas

  • General

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Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. / Narusaka, Mari; Toyoda, Kazuhiro; Shiraishi, Tomonori; Iuchi, Satoshi; Takano, Yoshitaka; Shirasu, Ken; Narusaka, Yoshihiro.

In: Scientific Reports, Vol. 6, 18702, 11.01.2016.

Research output: Contribution to journalArticle

Narusaka, Mari ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Iuchi, Satoshi ; Takano, Yoshitaka ; Shirasu, Ken ; Narusaka, Yoshihiro. / Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. In: Scientific Reports. 2016 ; Vol. 6.
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