Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio

Marek Zivcak, Klaudia Brückova, Oksana Sytar, Marian Brestic, Katarina Olsovska, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Main conclusion: Environmentally induced variation and the genotypic differences in flavonoid and phenolic content in lettuce can be reliably detected using the appropriate parameters derived from the records of rapid non-invasive fluorescence technique. The chlorophyll fluorescence excitation ratio method was designed as a rapid and non-invasive tool to estimate the content of UV-absorbing phenolic compounds in plants. Using this technique, we have assessed the dynamics of accumulation of flavonoids related to developmental changes and environmental effects. Moreover, we have tested appropriateness of the method to identify the genotypic differences and fluctuations in total phenolics and flavonoid content in lettuce. Six green and two red genotypes of lettuce (Lactuca sativa L.) grown in pots were exposed to two different environments for 50 days: direct sunlight (UV-exposed) and greenhouse conditions (low UV). The indices based on the measurements of chlorophyll fluorescence after red, green and UV excitation indicated increase of the content of UV-absorbing compounds and anthocyanins in the epidermis of lettuce leaves. In similar, the biochemical analyses performed at the end of the experiment confirmed significantly higher total phenolic and flavonoid content in lettuce plants exposed to direct sun compared to greenhouse conditions and in red compared to green genotypes. As the correlation between the standard fluorescence indices and the biochemical records was negatively influenced by the presence of red genotypes, we proposed the use of a new parameter named Modified Flavonoid Index (MFI) taking into an account both absorbance changes due to flavonol and anthocyanin content, for which the correlation with flavonoid and phenolic content was relatively good. Thus, our results confirmed that the fluorescence excitation ratio method is useful for identifying the major differences in phenolic and flavonoid content in lettuce plants and it can be used for high-throughput pre-screening and phenotyping of leafy vegetables in research and breeding applications towards improvement of vegetable health effects.

Original languageEnglish
Pages (from-to)1215-1229
Number of pages15
JournalPlanta
Volume245
Issue number6
DOIs
Publication statusPublished - Jun 1 2017
Externally publishedYes

Fingerprint

Lettuce
Chlorophyll
Flavonoids
lettuce
flavonoids
Fluorescence
fluorescence
screening
chlorophyll
phenotype
Anthocyanins
Genotype
Vegetables
anthocyanins
genotype
greenhouses
leaf lettuce
methodology
Sunlight
green leafy vegetables

Keywords

  • Chlorophyll fluorescence
  • Flavonoids
  • Lettuce
  • Phenolic
  • Phenotyping
  • UV radiation

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Zivcak, M., Brückova, K., Sytar, O., Brestic, M., Olsovska, K., & Allakhverdiev, S. (2017). Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio. Planta, 245(6), 1215-1229. https://doi.org/10.1007/s00425-017-2676-x

Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio. / Zivcak, Marek; Brückova, Klaudia; Sytar, Oksana; Brestic, Marian; Olsovska, Katarina; Allakhverdiev, Suleyman.

In: Planta, Vol. 245, No. 6, 01.06.2017, p. 1215-1229.

Research output: Contribution to journalArticle

Zivcak, M, Brückova, K, Sytar, O, Brestic, M, Olsovska, K & Allakhverdiev, S 2017, 'Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio', Planta, vol. 245, no. 6, pp. 1215-1229. https://doi.org/10.1007/s00425-017-2676-x
Zivcak M, Brückova K, Sytar O, Brestic M, Olsovska K, Allakhverdiev S. Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio. Planta. 2017 Jun 1;245(6):1215-1229. https://doi.org/10.1007/s00425-017-2676-x
Zivcak, Marek ; Brückova, Klaudia ; Sytar, Oksana ; Brestic, Marian ; Olsovska, Katarina ; Allakhverdiev, Suleyman. / Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio. In: Planta. 2017 ; Vol. 245, No. 6. pp. 1215-1229.
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