Less frequent promoter hypermethylation of DLC-1 gene in primary breast cancers

Atsushi Teramoto, Kazunori Tsukuda, Masaaki Yano, Shinichi Toyooka, Hideaki Dote, Hiroyoshi Doihara, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Absence or low expression of DLC-1, a tumor suppressor gene, in breast cancers has been shown recently. LOH of 8p12-p22, on which DLC-1 is located, is frequent in breast cancers, but the correlation between low expression of DLC-1 and LOH has not been confirmed. To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer-related genes, we examined the methylation status of DLC-1 promoter region in breast cancer cell lines and primary breast tumors. The hypermethylation status was examined by MSP and 25% of cell lines harbored a methylated allele. The gene silencing by methylation was also confirmed by the re-expression of DLC-1 by the 5-aza-2′-deoxycytidine treatment in DLC-1 hypermethylated cell line. But the methylation of DLC-1 gene was less frequently shown in primary breast cancers (10%). These data suggest that hypermethylation is responsible for silencing of DLC-1 gene in a limited portion of breast cancers.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalOncology reports
Volume12
Issue number1
DOIs
Publication statusPublished - Jul 2004

Keywords

  • Breast cancer
  • DLC-1
  • DNA methylation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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