Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus

Yoshimasa Hamada, Tetsuya Bando, Taro Nakamura, Yoshiyasu Ishimaru, Taro Mito, Sumihare Noji, Kenji Tomioka, Hideyo Ohuchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb’E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb’Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb’E(z)RNAi and was increased by Gb’UtxRNAi. Regenerated Gb’E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb’UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb’E(z)RNAi regenerating leg, the Gb’dac expression domain expanded in the tarsus. By contrast, in the Gb’UtxRNAi regenerating leg, Gb’Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression.

Original languageEnglish
Pages (from-to)2916-2927
Number of pages12
JournalDevelopment
Volume142
Issue number17
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Gryllidae
Histones
Methylation
Regeneration
Leg
Epigenomics
Ankle
RNA Interference
Gene Expression
X-Linked Genes
Tibia
Lysine
Insects
Joints

Keywords

  • Epigenetics
  • Gryllus bimaculatus
  • Histone H3K27
  • Polycomb
  • Regeneration

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus. / Hamada, Yoshimasa; Bando, Tetsuya; Nakamura, Taro; Ishimaru, Yoshiyasu; Mito, Taro; Noji, Sumihare; Tomioka, Kenji; Ohuchi, Hideyo.

In: Development, Vol. 142, No. 17, 01.09.2015, p. 2916-2927.

Research output: Contribution to journalArticle

Hamada, Yoshimasa ; Bando, Tetsuya ; Nakamura, Taro ; Ishimaru, Yoshiyasu ; Mito, Taro ; Noji, Sumihare ; Tomioka, Kenji ; Ohuchi, Hideyo. / Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus. In: Development. 2015 ; Vol. 142, No. 17. pp. 2916-2927.
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