Leg dysfunctions in a hatched chick model of spina bifida aperta

Katsumi Mominoki, Masae Kinutani, Hiroyuki Wakisaka, Shouichirou Saito, Naoto Kobayashi, Takashi Fujiwara, Seiji Matsuda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We created chicks with spina bifida aperta (SBA) by incising the roof plate of the neural tube of embryos at Hamburger and Hamilton stage 18 or 19. Incision over the length of three somites caused spina bifida occulta (SBO)-like malformation in 47% of the hatchlings. Incision over the length of five and seven somites caused SBA-like malformation in 100% of the hatchlings. The SBO chicks exhibited no symptoms, whereas the SBA chicks exhibited paralysis of a leg muscle and imbalance between an agonist and an antagonist leg muscles. Lesions in these SBA chicks were located in the spinal segments that give rise to motor neurons that innervated the dysfunctional muscles. Histological analysis revealed that there were fewer small spinal neurons (interneurons) at the site of the lesion in SBA chicks than in the normal chicks and that there was no such difference in the number of the large spinal neurons (motor neurons). Leg dysfunctions in this model of SBA may be attributable to the smaller number of interneurons in the spinal segments that contain motor neurons that innervate the dysfunctional muscle. This model may facilitate studies of the pathological mechanisms that lead to leg dysfunctions in SBA chicks.

Original languageEnglish
Pages (from-to)133-142
Number of pages10
JournalExperimental Neurology
Volume197
Issue number1
DOIs
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Spina Bifida Cystica
Leg
Motor Neurons
Spina Bifida Occulta
Muscles
Somites
Interneurons
Neurons
Neural Tube
Paraplegia
Embryonic Structures

Keywords

  • Chick
  • Leg dysfunction
  • Neural tube defects
  • Spina bifida model

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Mominoki, K., Kinutani, M., Wakisaka, H., Saito, S., Kobayashi, N., Fujiwara, T., & Matsuda, S. (2006). Leg dysfunctions in a hatched chick model of spina bifida aperta. Experimental Neurology, 197(1), 133-142. https://doi.org/10.1016/j.expneurol.2005.09.001

Leg dysfunctions in a hatched chick model of spina bifida aperta. / Mominoki, Katsumi; Kinutani, Masae; Wakisaka, Hiroyuki; Saito, Shouichirou; Kobayashi, Naoto; Fujiwara, Takashi; Matsuda, Seiji.

In: Experimental Neurology, Vol. 197, No. 1, 01.2006, p. 133-142.

Research output: Contribution to journalArticle

Mominoki, K, Kinutani, M, Wakisaka, H, Saito, S, Kobayashi, N, Fujiwara, T & Matsuda, S 2006, 'Leg dysfunctions in a hatched chick model of spina bifida aperta', Experimental Neurology, vol. 197, no. 1, pp. 133-142. https://doi.org/10.1016/j.expneurol.2005.09.001
Mominoki, Katsumi ; Kinutani, Masae ; Wakisaka, Hiroyuki ; Saito, Shouichirou ; Kobayashi, Naoto ; Fujiwara, Takashi ; Matsuda, Seiji. / Leg dysfunctions in a hatched chick model of spina bifida aperta. In: Experimental Neurology. 2006 ; Vol. 197, No. 1. pp. 133-142.
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