Abstract
Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 584-588 |
| Number of pages | 5 |
| Journal | Virchows Archiv |
| Volume | 441 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Con A
- Cytochemistry
- LCA
- PSA
- Reactive astrocyte
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Lectin cytochemical demonstration of glucose- and mannose-containing glycoconjugates on human reactive astrocytes. / Kawai, Kensuke; Terada, Seishi; Yokota, Osamu; Fujita, Daisuke; Ishizu, Hideki; Kuroda, Shigetoshi.
In: Virchows Archiv, Vol. 441, No. 6, 2002, p. 584-588.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lectin cytochemical demonstration of glucose- and mannose-containing glycoconjugates on human reactive astrocytes
AU - Kawai, Kensuke
AU - Terada, Seishi
AU - Yokota, Osamu
AU - Fujita, Daisuke
AU - Ishizu, Hideki
AU - Kuroda, Shigetoshi
PY - 2002
Y1 - 2002
N2 - Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.
AB - Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.
KW - Con A
KW - Cytochemistry
KW - LCA
KW - PSA
KW - Reactive astrocyte
UR - http://www.scopus.com/inward/record.url?scp=0036454173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036454173&partnerID=8YFLogxK
U2 - 10.1007/s00428-002-0636-7
DO - 10.1007/s00428-002-0636-7
M3 - Article
VL - 441
SP - 584
EP - 588
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 6
ER -