Lectin cytochemical demonstration of glucose- and mannose-containing glycoconjugates on human reactive astrocytes

Kensuke Kawai; Seishi Terada; Osamu Yokota; Daisuke Fujita; Hideki Ishizu; Shigetoshi Kuroda

doi:10.1007/s00428-002-0636-7

Lectin cytochemical demonstration of glucose- and mannose-containing glycoconjugates on human reactive astrocytes

Kensuke Kawai, Seishi Terada, Osamu Yokota, Daisuke Fujita, Hideki Ishizu, Shigetoshi Kuroda

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.

Original language	English
Pages (from-to)	584-588
Number of pages	5
Journal	Virchows Archiv
Volume	441
Issue number	6
DOIs	https://doi.org/10.1007/s00428-002-0636-7
Publication status	Published - 2002

Keywords

Con A
Cytochemistry
LCA
PSA
Reactive astrocyte

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

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Kawai, K., Terada, S., Yokota, O., Fujita, D., Ishizu, H., & Kuroda, S. (2002). Lectin cytochemical demonstration of glucose- and mannose-containing glycoconjugates on human reactive astrocytes. Virchows Archiv, 441(6), 584-588. https://doi.org/10.1007/s00428-002-0636-7

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abstract = "Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.",

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AU - Kawai, Kensuke

AU - Terada, Seishi

AU - Yokota, Osamu

AU - Fujita, Daisuke

AU - Ishizu, Hideki

AU - Kuroda, Shigetoshi

PY - 2002

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N2 - Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.

AB - Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.

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