Lectin cytochemical demonstration of glucose- and mannose-containing glycoconjugates on human reactive astrocytes

Kensuke Kawai, Seishi Terada, Osamu Yokota, Daisuke Fujita, Hideki Ishizu, Shigetoshi Kuroda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lectin cytochemistry of the human autopsy cases of Creutzfeldt-Jakob disease and old cerebral infarction was performed on formalin-fixed, paraffin-embedded sections using 15 lectins. Reactive astrocytes showed a positive reaction with concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Pisum sativum agglutinin (PSA), with common specificity for both mannose and glucose. However normal astrocytes demonstrated no or little cytochemical reaction for any of the lectins. Reactivity for the lectins in control sections was obviously reduced using the blocking sugars (0.1 mol/lD-mannose and 0.5 mol/lD-glucose for Con A, LCA, and PSA, and 0.5 M mol/l α-methyl mannopy-ranoside for Con A). The present data provide suggestive evidence that the reactivity for Con A, LCA, and PSA is increased in reactive astrocytes.

Original languageEnglish
Pages (from-to)584-588
Number of pages5
JournalVirchows Archiv
Volume441
Issue number6
DOIs
Publication statusPublished - 2002

Keywords

  • Con A
  • Cytochemistry
  • LCA
  • PSA
  • Reactive astrocyte

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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