Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices

Hiroshi Ueno, Shunsuke Suemitsu, Shinji Murakami, Naoya Kitamura, Kenta Wani, Yousuke Matsumoto, Motoi Okamoto, Takeshi Ishihara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the developing central nervous system (CNS), extracellular matrix (ECM) molecules have regulating roles such as in brain development, neural-circuit maturation, and synaptic-function control. However, excluding the perineuronal net (PNN) area, the distribution, constituent elements, and expression level of granular ECM molecules (diffuse ECM) present in the mature CNS remain unclear. Diffuse ECM molecules in the CNS share the components of PNNs and are likely functional. As cortical functions are greatly region-dependent, we hypothesized that ECM molecules would differ in distribution, expression level, and components in a region- and layer-dependent manner. We examined the layer-specific expression of several chondroitin sulfate proteoglycans (aggrecan, neurocan, and brevican), tenascin-R, Wisteria floribunda agglutinin (WFA)-positive molecules, hyaluronic acid, and link protein in the somatosensory and piriform cortices of mature mice. Furthermore, we investigated expression changes in WFA-positive molecules due to aging. In the somatosensory cortex, PNN density was particularly high at layer 4 (L4), but not all diffuse ECM molecules were highly expressed at L4 compared to the other layers. There was almost no change in tenascin-R and hyaluronic acid in any somatosensory-cortex layer. Neurocan showed high expression in L1 of the somatosensory cortex. In the piriform cortex, many ECM molecules showed higher expression in L1 than in the other layers. However, hyaluronic acid showed high expression in deep layers. Here, we clarified that ECM molecules differ in constituent elements and expression in a region- and layer-dependent manner. Region-specific expression of ECM molecules is possibly related to functions such as region-specific plasticity and vulnerability.

Original languageEnglish
Pages (from-to)1-17
Number of pages17
JournalIBRO Reports
Volume6
DOIs
Publication statusPublished - Jun 1 2019

Fingerprint

Somatosensory Cortex
Extracellular Matrix
Neurocan
Hyaluronic Acid
Central Nervous System
Brevican
Chondroitin Sulfate Proteoglycans
Piriform Cortex
Aggrecans
Brain

Keywords

  • Extracellular matrix
  • Perineuronal nets
  • Piriform cortex
  • Proteoglycans
  • Somatosensory cortex
  • Wisteria floribunda

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices. / Ueno, Hiroshi; Suemitsu, Shunsuke; Murakami, Shinji; Kitamura, Naoya; Wani, Kenta; Matsumoto, Yousuke; Okamoto, Motoi; Ishihara, Takeshi.

In: IBRO Reports, Vol. 6, 01.06.2019, p. 1-17.

Research output: Contribution to journalArticle

Ueno, Hiroshi ; Suemitsu, Shunsuke ; Murakami, Shinji ; Kitamura, Naoya ; Wani, Kenta ; Matsumoto, Yousuke ; Okamoto, Motoi ; Ishihara, Takeshi. / Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices. In: IBRO Reports. 2019 ; Vol. 6. pp. 1-17.
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AU - Kitamura, Naoya

AU - Wani, Kenta

AU - Matsumoto, Yousuke

AU - Okamoto, Motoi

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