@article{eef1802ee7e148c28e4dc1490c15fc32,
title = "Lattice-patterned collagen fibers and their dynamics in axolotl skin regeneration",
abstract = "The morphology of collagen-producing cells and the structure of produced collagen in the dermis have not been well-described. This lack of insights has been a serious obstacle in the evaluation of skin regeneration. We succeeded in visualizing collagen-producing cells and produced collagen using the axolotl skin, which is highly transparent. The visualized dermal collagen had a lattice-like structure. The collagen-producing fibroblasts consistently possessed the lattice-patterned filopodia along with the lattice-patterned collagen network. The dynamics of this lattice-like structure were also verified in the skin regeneration process of axolotls, and it was found that the correct lattice-like structure was not reorganized after simple skin wounding but was reorganized in the presence of nerves. These findings are not only fundamental insights in dermatology but also valuable insights into the mechanism of skin regeneration.",
keywords = "Animal physiology, Bioengineering, Physiology",
author = "Rena Kashimoto and Saya Furukawa and Sakiya Yamamoto and Yasuhiro Kamei and Joe Sakamoto and Shigenori Nonaka and Watanabe, {Tomonobu M.} and Tatsuya Sakamoto and Hirotaka Sakamoto and Akira Satoh",
note = "Funding Information: We are grateful to R. Iwata and T. Satoh for their laboratory work. We thank Dr. Kaneshiro for his critical discussion and some trial experiments. We received intangible support from Olympus in our early works on collagen imaging, for which we are grateful to Mr. Takeshita (Okuma/Olympus). We also thank Dr. S. Aizawa for rat skin preparation. We thank the NIBB Bioimage Analysis Training Course 2021 (sponsored by ABiS: JSPS KAKENHI Grant Number JP16H06280 ) for teaching basic knowledge of image analysis. Axolotls were obtained through Hiroshima University Amphibian Research Funding. This work is supported by a JSPS KAKENHI grant-in-aid for scientific research (B) (# 20H03264 to AS), JST SPRING (# JPMJSP2126 to RK), Koyanagi Foundation (to AS), and NIBB Collaborative Research Program for integrative imaging ( 20-514 and 21-411 to YK and AS). Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jul,
day = "15",
doi = "10.1016/j.isci.2022.104524",
language = "English",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "7",
}