Lats2 is an essential mitotic regulator required for the coordination of cell division

Norikazu Yabuta, Nobuhiro Okada, Akihiko Ito, Toshiya Hosomi, Souichi Nishihara, Yuya Sasayama, Azumi Fujimori, Daisuke Okuzaki, Hanjun Zhao, Masahito Ikawa, Masaru Okabe, Hiroshi Nojima

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Tumor suppressor Lats2 is a member of the conserved Dbf2 kinase family. It localizes to the centrosome and has been implicated in regulation of the cell cycle and apoptosis. However, the in vivo function of this kinase remains unclear. Here, we show that complete disruption of the gene encoding Lats2 in mice causes developmental defects in the nervous system and embryonic lethality. Furthermore, mutant cells derived from total LATS2-knock-out embryos exhibit mitotic defects including centrosome fragmentation and cytokinesis defects, followed by nuclear enlargement and multinucleation. We show that the Mob1 family, a regulator of mitotic exit, associates with Lats2 to induce its activation. We also show that the complete LATS2-knock-out cells exhibit an acceleration of exit from mitosis and marked down-regulation of critical mitotic regulators. These results suggest that Lats2 plays an essential mitotic role in coordinating accurate cytokinesis completion, governing the stabilization of other mitotic regulators.

Original languageEnglish
Pages (from-to)19259-19271
Number of pages13
JournalJournal of Biological Chemistry
Volume282
Issue number26
DOIs
Publication statusPublished - Jun 29 2007
Externally publishedYes

Fingerprint

Centrosome
Cytokinesis
Cell Division
Phosphotransferases
Cells
Defects
Mitosis
Nervous System
Cell Cycle
Gene encoding
Down-Regulation
Embryonic Structures
Neurology
Apoptosis
Tumors
Stabilization
Chemical activation
Genes
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yabuta, N., Okada, N., Ito, A., Hosomi, T., Nishihara, S., Sasayama, Y., ... Nojima, H. (2007). Lats2 is an essential mitotic regulator required for the coordination of cell division. Journal of Biological Chemistry, 282(26), 19259-19271. https://doi.org/10.1074/jbc.M608562200

Lats2 is an essential mitotic regulator required for the coordination of cell division. / Yabuta, Norikazu; Okada, Nobuhiro; Ito, Akihiko; Hosomi, Toshiya; Nishihara, Souichi; Sasayama, Yuya; Fujimori, Azumi; Okuzaki, Daisuke; Zhao, Hanjun; Ikawa, Masahito; Okabe, Masaru; Nojima, Hiroshi.

In: Journal of Biological Chemistry, Vol. 282, No. 26, 29.06.2007, p. 19259-19271.

Research output: Contribution to journalArticle

Yabuta, N, Okada, N, Ito, A, Hosomi, T, Nishihara, S, Sasayama, Y, Fujimori, A, Okuzaki, D, Zhao, H, Ikawa, M, Okabe, M & Nojima, H 2007, 'Lats2 is an essential mitotic regulator required for the coordination of cell division', Journal of Biological Chemistry, vol. 282, no. 26, pp. 19259-19271. https://doi.org/10.1074/jbc.M608562200
Yabuta, Norikazu ; Okada, Nobuhiro ; Ito, Akihiko ; Hosomi, Toshiya ; Nishihara, Souichi ; Sasayama, Yuya ; Fujimori, Azumi ; Okuzaki, Daisuke ; Zhao, Hanjun ; Ikawa, Masahito ; Okabe, Masaru ; Nojima, Hiroshi. / Lats2 is an essential mitotic regulator required for the coordination of cell division. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 26. pp. 19259-19271.
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