Laser-induced propagation and destruction of amyloid β fibrils

Hisashi Yagi, Daisaku Ozawa, Kazumasa Sakurai, Toru Kawakami, Hiroki Kuyama, Osamu Nishimura, Toshinori Shimanouchi, Ryoichi Kuboi, Hironobu Naiki, Yuji Goto

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The amyloid deposition of amyloid β(Aβ) peptides is a critical pathological event in Alzheimer disease (AD). Preventing the formation of amyloid deposits and removing preformed fibrils in tissues are important therapeutic strategies against AD. Previously, we reported the destruction of amyloid fibrils of β2-microglobulin K3 fragments by laser irradiation coupled with the binding of amyloid-specific thioflavin T. Here, we studied the effects of a laser beam on Aβ fibrils. As was the case for K3 fibrils, extensive irradiation destroyed the preformed Aβ fibrils. However, irradiation during spontaneous fibril formation resulted in only the partial destruction of growing fibrils and a subsequent explosive propagation of fibrils. The explosive propagation was caused by an increase in the number of active ends due to breakage. The results not only reveal a case of fragmentation-induced propagation of fibrils but also provide insights into therapeutic strategies for AD.

Original languageEnglish
Pages (from-to)19660-19667
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number25
DOIs
Publication statusPublished - Jun 18 2010

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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