Laryngeal squamous cell papilloma is highly associated with human papillomavirus

Yorihisa Orita, Yuka Gion, Tomoyasu Tachibana, Kana Ikegami, Hidenori Marunaka, Seiichiro Makihara, Yasuhiko Yamashita, Kentaro Miki, Takuma Makino, Naoki Akisada, Yusuke Akagi, Miyuki Kimura, Tadashi Yoshino, Kazunori Nishizaki, Yasuharu Sato

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Abstract

Objective: To delineate the association between characteristics of adult-onset laryngeal squamous cell papilloma and human papillomavirus (HPV) infection. Methods: Clinical records and paraffin-embedded specimens of 77 papilloma patients who had been treated between 1998 and 2014 were collected. Of the 77 cases, 34 were identified in the larynx, 28 in the oral cavity and 15 in the oropharynx. Specimens were investigated by polymerase chain reaction (PCR) to detect HPV 6, 11, 16, 18, 31, 33, 35, 52b and 58, and immunohistochemical (IHC) staining for anti-p16INK4a antibody. Results: In 21 cases (61.8%) with laryngeal squamous cell papilloma, various types of HPV were detected: 14 cases (41.2%) were positive of high-risk HPV, 18 (52.9%) were positive of low-risk HPV and 11 (32.4%) were positive of both high-risk HPV and low-risk HPV. Younger patients (<60 years) showed a higher rate of HPV infection than older patients. Among the 34 cases with laryngeal papilloma, no malignant transformation was observed during the study period. With IHC staining, positive expression of p16 was observed in 20 cases (58.8%). HPV infection and p16-expression were associated with the pathological finding of koilocytosis. Only four cases (14.3%) showed HPV-positivity in the oral cavity, and none of the 15 oropharyngeal cases were positive for HPV, and none of the oral cavity and oropharyngeal cases showed koilocytosis. Results of HPV-PCR and p16-IHC staining were significantly correlated each other. Conclusions: HPV infection is frequently associated with laryngeal squamous cell papilloma, and koilocytosis is a characteristic pathological finding. To the best of our knowledge, this is the first report which have described infections with multiple HPV types in laryngeal papilloma.

Original languageEnglish
Pages (from-to)350-355
Number of pages6
JournalJapanese Journal of Clinical Oncology
Volume48
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Papilloma
Papillomavirus Infections
Human papillomavirus 11
Mouth
Staining and Labeling
Human papillomavirus 6
Human papillomavirus 18
Polymerase Chain Reaction
Oropharynx
Larynx
Paraffin
Anti-Idiotypic Antibodies

Keywords

  • Human papillomavirus
  • Larynx
  • Oral cavity
  • P16
  • Pharynx

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Laryngeal squamous cell papilloma is highly associated with human papillomavirus. / Orita, Yorihisa; Gion, Yuka; Tachibana, Tomoyasu; Ikegami, Kana; Marunaka, Hidenori; Makihara, Seiichiro; Yamashita, Yasuhiko; Miki, Kentaro; Makino, Takuma; Akisada, Naoki; Akagi, Yusuke; Kimura, Miyuki; Yoshino, Tadashi; Nishizaki, Kazunori; Sato, Yasuharu.

In: Japanese Journal of Clinical Oncology, Vol. 48, No. 4, 01.04.2018, p. 350-355.

Research output: Contribution to journalArticle

Orita, Yorihisa ; Gion, Yuka ; Tachibana, Tomoyasu ; Ikegami, Kana ; Marunaka, Hidenori ; Makihara, Seiichiro ; Yamashita, Yasuhiko ; Miki, Kentaro ; Makino, Takuma ; Akisada, Naoki ; Akagi, Yusuke ; Kimura, Miyuki ; Yoshino, Tadashi ; Nishizaki, Kazunori ; Sato, Yasuharu. / Laryngeal squamous cell papilloma is highly associated with human papillomavirus. In: Japanese Journal of Clinical Oncology. 2018 ; Vol. 48, No. 4. pp. 350-355.
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abstract = "Objective: To delineate the association between characteristics of adult-onset laryngeal squamous cell papilloma and human papillomavirus (HPV) infection. Methods: Clinical records and paraffin-embedded specimens of 77 papilloma patients who had been treated between 1998 and 2014 were collected. Of the 77 cases, 34 were identified in the larynx, 28 in the oral cavity and 15 in the oropharynx. Specimens were investigated by polymerase chain reaction (PCR) to detect HPV 6, 11, 16, 18, 31, 33, 35, 52b and 58, and immunohistochemical (IHC) staining for anti-p16INK4a antibody. Results: In 21 cases (61.8{\%}) with laryngeal squamous cell papilloma, various types of HPV were detected: 14 cases (41.2{\%}) were positive of high-risk HPV, 18 (52.9{\%}) were positive of low-risk HPV and 11 (32.4{\%}) were positive of both high-risk HPV and low-risk HPV. Younger patients (<60 years) showed a higher rate of HPV infection than older patients. Among the 34 cases with laryngeal papilloma, no malignant transformation was observed during the study period. With IHC staining, positive expression of p16 was observed in 20 cases (58.8{\%}). HPV infection and p16-expression were associated with the pathological finding of koilocytosis. Only four cases (14.3{\%}) showed HPV-positivity in the oral cavity, and none of the 15 oropharyngeal cases were positive for HPV, and none of the oral cavity and oropharyngeal cases showed koilocytosis. Results of HPV-PCR and p16-IHC staining were significantly correlated each other. Conclusions: HPV infection is frequently associated with laryngeal squamous cell papilloma, and koilocytosis is a characteristic pathological finding. To the best of our knowledge, this is the first report which have described infections with multiple HPV types in laryngeal papilloma.",
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T1 - Laryngeal squamous cell papilloma is highly associated with human papillomavirus

AU - Orita, Yorihisa

AU - Gion, Yuka

AU - Tachibana, Tomoyasu

AU - Ikegami, Kana

AU - Marunaka, Hidenori

AU - Makihara, Seiichiro

AU - Yamashita, Yasuhiko

AU - Miki, Kentaro

AU - Makino, Takuma

AU - Akisada, Naoki

AU - Akagi, Yusuke

AU - Kimura, Miyuki

AU - Yoshino, Tadashi

AU - Nishizaki, Kazunori

AU - Sato, Yasuharu

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N2 - Objective: To delineate the association between characteristics of adult-onset laryngeal squamous cell papilloma and human papillomavirus (HPV) infection. Methods: Clinical records and paraffin-embedded specimens of 77 papilloma patients who had been treated between 1998 and 2014 were collected. Of the 77 cases, 34 were identified in the larynx, 28 in the oral cavity and 15 in the oropharynx. Specimens were investigated by polymerase chain reaction (PCR) to detect HPV 6, 11, 16, 18, 31, 33, 35, 52b and 58, and immunohistochemical (IHC) staining for anti-p16INK4a antibody. Results: In 21 cases (61.8%) with laryngeal squamous cell papilloma, various types of HPV were detected: 14 cases (41.2%) were positive of high-risk HPV, 18 (52.9%) were positive of low-risk HPV and 11 (32.4%) were positive of both high-risk HPV and low-risk HPV. Younger patients (<60 years) showed a higher rate of HPV infection than older patients. Among the 34 cases with laryngeal papilloma, no malignant transformation was observed during the study period. With IHC staining, positive expression of p16 was observed in 20 cases (58.8%). HPV infection and p16-expression were associated with the pathological finding of koilocytosis. Only four cases (14.3%) showed HPV-positivity in the oral cavity, and none of the 15 oropharyngeal cases were positive for HPV, and none of the oral cavity and oropharyngeal cases showed koilocytosis. Results of HPV-PCR and p16-IHC staining were significantly correlated each other. Conclusions: HPV infection is frequently associated with laryngeal squamous cell papilloma, and koilocytosis is a characteristic pathological finding. To the best of our knowledge, this is the first report which have described infections with multiple HPV types in laryngeal papilloma.

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KW - Human papillomavirus

KW - Larynx

KW - Oral cavity

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