Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel

Ryota Takeuchi, Megumi Maeda, Miran Nakano, Hiroaki Funahashi, Yoshinobu Kimura

Research output: Contribution to journalArticlepeer-review

Abstract

Sialyl-Tn antigen, a tumor antigen, is a valuable ligand for the purification of proteins that specifically bind to it. Here, we developed a new method for the preparation of large amounts of sialyl-Tn antigen-containing peptides from an unused resource, boar seminal gel. The glycopeptides were prepared from the actinase E digests by a combination of gel filtration and hydrophilic partitioning.

Original languageEnglish
Pages (from-to)2022-2025
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume85
Issue number9
DOIs
Publication statusPublished - Sep 1 2021

Keywords

  • O-glycans
  • boar seminal gel
  • mucin-like glycoproteins
  • sialyl-Tn antigens

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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