Large-scale preparation of glycopeptides harboring the TF-antigen unit from royal jelly

Megumi Maeda; Tatsuya Tanaka; Mariko Kimura; Yoshinobu Kimura

doi:10.1080/09168451.2014.878223

Large-scale preparation of glycopeptides harboring the TF-antigen unit from royal jelly

Megumi Maeda, Tatsuya Tanaka, Mariko Kimura, Yoshinobu Kimura

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We have reported that new N-glycans carrying the TF-antigen occurred on a major royal jelly glycoprotein, and we have identified the glycosylation site to which the antigenic N-glycan is linked, but an appropriate procedure has not been established to prepare non-labeled immunoreactive glycopeptides in large amounts for functional analysis. In this study, we developed an effective method of preparing Asn-glycopeptide bearing TF-antigen.

Original language	English
Pages (from-to)	276-278
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	78
Issue number	2
DOIs	https://doi.org/10.1080/09168451.2014.878223
Publication status	Published - 2014

Keywords

Glycopeptide preparation
Immunoreactive glycan
Insect N-glycan
Royal jelly
TF-antigen unit

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2014.878223

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title = "Large-scale preparation of glycopeptides harboring the TF-antigen unit from royal jelly",

abstract = "We have reported that new N-glycans carrying the TF-antigen occurred on a major royal jelly glycoprotein, and we have identified the glycosylation site to which the antigenic N-glycan is linked, but an appropriate procedure has not been established to prepare non-labeled immunoreactive glycopeptides in large amounts for functional analysis. In this study, we developed an effective method of preparing Asn-glycopeptide bearing TF-antigen.",

keywords = "Glycopeptide preparation, Immunoreactive glycan, Insect N-glycan, Royal jelly, TF-antigen unit",

author = "Megumi Maeda and Tatsuya Tanaka and Mariko Kimura and Yoshinobu Kimura",

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doi = "10.1080/09168451.2014.878223",

language = "English",

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AU - Maeda, Megumi

AU - Tanaka, Tatsuya

AU - Kimura, Mariko

AU - Kimura, Yoshinobu

N1 - Funding Information: This work was supported by Basic Research C from the Ministry of Education, Culture, Sports, Science and Technology of Japan [24580494 to M. M., 24580495 to Y. K., 23400944 to M. K.], and Program to disseminate tenure tracking system from the Ministry of Education, Culture, Sports, Science and Technology of Japan [FY 2011-2013 to M. M.]. Publisher Copyright: © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

PY - 2014

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N2 - We have reported that new N-glycans carrying the TF-antigen occurred on a major royal jelly glycoprotein, and we have identified the glycosylation site to which the antigenic N-glycan is linked, but an appropriate procedure has not been established to prepare non-labeled immunoreactive glycopeptides in large amounts for functional analysis. In this study, we developed an effective method of preparing Asn-glycopeptide bearing TF-antigen.

AB - We have reported that new N-glycans carrying the TF-antigen occurred on a major royal jelly glycoprotein, and we have identified the glycosylation site to which the antigenic N-glycan is linked, but an appropriate procedure has not been established to prepare non-labeled immunoreactive glycopeptides in large amounts for functional analysis. In this study, we developed an effective method of preparing Asn-glycopeptide bearing TF-antigen.

KW - Glycopeptide preparation

KW - Immunoreactive glycan

KW - Insect N-glycan

KW - Royal jelly

KW - TF-antigen unit

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Large-scale preparation of glycopeptides harboring the TF-antigen unit from royal jelly

Abstract

Keywords

ASJC Scopus subject areas

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