Large-scale preparation of Asn-glycopeptide carrying structurally homologous antigenic N-glycan

Megumi Maeda, Naoto Takeda, Aya Mano, Maomi Yamanishi, Mariko Kimura, Yoshinobu Kimura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770-778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asnglycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.

Original languageEnglish
Pages (from-to)1269-1274
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number6
DOIs
Publication statusPublished - Jul 19 2013

Keywords

  • Ginkgo biloba
  • Glycopeptide preparation
  • Immunoreactive glycan
  • Neoglycoconjugate
  • Plant N-glycan

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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