Large inversion in Escherichia coli K-12 1485IN between inversely oriented IS3 elements near lac and cdd

Y. Komoda, M. Enomoto, Akira Tominaga

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15 Citations (Scopus)

Abstract

A companion study has shown that the inversion carried by strain 1485IN has one terminus between lac and proC and the other between his and cdd of the normal strain. Starting with this mapping data, we have done molecular work demonstrating that the inversion occurred by recombination between inversely oriented two IS3 elements, one present near lac and the other near the cdd locus; i.e., the inversion is IN(is3B-is3E). Evidence supporting this conclusion includes: (i) Normal and inversion strains share two short regions with identical restriction maps. One of these regions is near lac and the other near cdd. (ii) IS3 homology was detected in each of the terminus regions of both the normal and inversion strains. (iii) The sequence on one side of the original IS3 element near lac has been exchanged with the sequence on one side of the IS3 near cdd. Whether the inversion has occurred by one event of homologous recombination between the two IS3 elements or has been caused by involvement of IS3 elements on an F factor is discussed. Another rearrangement, probably related to inversion and deletion, was detected between the IS3 and cdd of the inversion strain.

Original languageEnglish
Pages (from-to)639-645
Number of pages7
JournalGenetics
Volume129
Issue number3
Publication statusPublished - 1991

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Homologous Recombination
Genetic Recombination
Escherichia coli

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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Large inversion in Escherichia coli K-12 1485IN between inversely oriented IS3 elements near lac and cdd. / Komoda, Y.; Enomoto, M.; Tominaga, Akira.

In: Genetics, Vol. 129, No. 3, 1991, p. 639-645.

Research output: Contribution to journalArticle

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N2 - A companion study has shown that the inversion carried by strain 1485IN has one terminus between lac and proC and the other between his and cdd of the normal strain. Starting with this mapping data, we have done molecular work demonstrating that the inversion occurred by recombination between inversely oriented two IS3 elements, one present near lac and the other near the cdd locus; i.e., the inversion is IN(is3B-is3E). Evidence supporting this conclusion includes: (i) Normal and inversion strains share two short regions with identical restriction maps. One of these regions is near lac and the other near cdd. (ii) IS3 homology was detected in each of the terminus regions of both the normal and inversion strains. (iii) The sequence on one side of the original IS3 element near lac has been exchanged with the sequence on one side of the IS3 near cdd. Whether the inversion has occurred by one event of homologous recombination between the two IS3 elements or has been caused by involvement of IS3 elements on an F factor is discussed. Another rearrangement, probably related to inversion and deletion, was detected between the IS3 and cdd of the inversion strain.

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