LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice

Wan Yi Chiou, Tadafumi Kawamoto, Eiko Himi, Kazuhide Rikiishi, Manabu Sugimoto, Mika Hayashi-Tsugane, Kazuo Tsugane, Masahiko Maekawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Grain size is a key determiner of grain weight, one of the yield components in rice (Oryza sativa). Therefore, to increase grain yield, it is important to elucidate the detailed mechanisms regulating grain size. The Large grain (Lgg) mutant, found in the nonautonomous DNA-based active rice transposon1 (nDart1)-tagged lines of Koshihikari, is caused by a truncated nDart1-3 and 355 bp deletion in the 5′ untranslated region of LGG, which encodes a putative RNA-binding protein, through transposon display and cosegregation analysis between grain length and LGG genotype in F2 and F3. Clustered regularly interspaced short palindromic repeats/CRISPR-associated 9-mediated knockout and overexpression of LGG led to longer and shorter grains than wild type, respectively, showing that LGG regulates spikelet hull length. Expression of LGG was highest in the 0.6-mm-long young panicle and gradually decreased as the panicle elongated. LGG was also expressed in roots and leaves. These results show that LGG functions at the very early stage of panicle development. Longitudinal cell numbers of spikelet hulls of Lgg, knockout and overexpressed plants were significantly different from those of the wild type, suggesting that LGG might regulate longitudinal cell proliferation in the spikelet hull. RNA-Seq analysis of 1-mm-long young panicles from LGG knockout and overexpressing plants revealed that the expressions of many cell cycle-related genes were reduced in knockout plants relative to LGG-overexpressing plants and wild type, whereas some genes for cell proliferation were highly expressed in knockout plants. Taken together, these results suggest that LGG might be a regulator of cell cycle and cell division in the rice spikelet hull.

Original languageEnglish
Pages (from-to)503-515
Number of pages13
JournalPlant and Cell Physiology
Volume60
Issue number3
DOIs
Publication statusPublished - Mar 1 2019

Fingerprint

RNA-binding proteins
RNA-Binding Proteins
hulls
inflorescences
rice
Clustered Regularly Interspaced Short Palindromic Repeats
Cell Proliferation
cdc Genes
cell cycle
cell proliferation
5' Untranslated Regions
DNA
Cell Division
Cell Cycle
5' untranslated regions
Cell Count
Genotype
Oryza
RNA
transposons

Keywords

  • DNA transposon
  • Grain size
  • Oryza sativa
  • RNA-binding protein
  • Tagged lines

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice. / Chiou, Wan Yi; Kawamoto, Tadafumi; Himi, Eiko; Rikiishi, Kazuhide; Sugimoto, Manabu; Hayashi-Tsugane, Mika; Tsugane, Kazuo; Maekawa, Masahiko.

In: Plant and Cell Physiology, Vol. 60, No. 3, 01.03.2019, p. 503-515.

Research output: Contribution to journalArticle

Chiou, Wan Yi ; Kawamoto, Tadafumi ; Himi, Eiko ; Rikiishi, Kazuhide ; Sugimoto, Manabu ; Hayashi-Tsugane, Mika ; Tsugane, Kazuo ; Maekawa, Masahiko. / LARGE GRAIN Encodes a Putative RNA-Binding Protein that Regulates Spikelet Hull Length in Rice. In: Plant and Cell Physiology. 2019 ; Vol. 60, No. 3. pp. 503-515.
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AU - Himi, Eiko

AU - Rikiishi, Kazuhide

AU - Sugimoto, Manabu

AU - Hayashi-Tsugane, Mika

AU - Tsugane, Kazuo

AU - Maekawa, Masahiko

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