Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA110

Lun Wang, Soya Suganuma, Ayumi Hibino, Ryoji Mitsui, Akio Tani, Takashi Matsumoto, Akio Ebihara, Nanung Agus Fitriyanto, Ambar Pertiwiningrum, Masaya Shimada, Takashi Hayakawa, Tomoyuki Nakagawa

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4 Citations (Scopus)

Abstract

The legume symbiotic nitrogen-fixing bacterium, B. diazoefficiens strain USDA110, utilizes methanol for growth in the presence of light lanthanides, such as La3+, Ce3+, Pr3+ or Nd3+, and its cells possess significant methanol dehydrogenase (MDH) activity. We purified MDH to homogeneity from B. diazoefficiens strain USDA110 grown in a methanol/Ce3+ medium; the protein was identified as XoxF5-type MDH (blr6213). The purified XoxF contained 0.58 cerium atoms per enzyme subunit. Moreover, the in-solution structure of XoxF was analyzed by small angle X-ray scattering (SAXS) analysis; the radius of gyration (Rg) and maximum particle dimension (Dmax) of XoxF were calculated to be 32.3 and 96.8 Å, respectively, suggesting that XoxF adopts a dimer structure in solution. These results show that B. diazoefficiens strain USDA110 has XoxF, a lanthanides-dependent MDH, which has methanol oxidation activity and is induced by methanol/lanthanaides, and that lanthanide is one of the important factors in methanol utilization by the strain.

Original languageEnglish
Article number109371
JournalEnzyme and Microbial Technology
Volume130
DOIs
Publication statusPublished - Nov 2019

Keywords

  • Bradyrhizobium diazoefficiens
  • Lanthanide, XoxF
  • Methanol dehydrogenase
  • Methanol utilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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    Wang, L., Suganuma, S., Hibino, A., Mitsui, R., Tani, A., Matsumoto, T., Ebihara, A., Fitriyanto, N. A., Pertiwiningrum, A., Shimada, M., Hayakawa, T., & Nakagawa, T. (2019). Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA110. Enzyme and Microbial Technology, 130, [109371]. https://doi.org/10.1016/j.enzmictec.2019.109371