Lanthanide-dependent methanol and formaldehyde oxidation in methylobacterium aquaticum strain 22A

Patcha Yanpirat, Yukari Nakatsuji, Shota Hiraga, Yoshiko Fujitani, Terumi Izumi, Sachiko Masuda, Ryoji Mitsui, Tomoyuki Nakagawa, Akio Tani

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Lanthanides (Ln) are an essential cofactor for XoxF-type methanol dehydrogenases (MDHs) in Gram-negative methylotrophs. The Ln3+ dependency of XoxF has expanded knowledge and raised new questions in methylotrophy, including the differences in characteristics of XoxF-type MDHs, their regulation, and the methylotrophic metabolism including formaldehyde oxidation. In this study, we genetically identified one set of Ln3+-and Ca2+-dependent MDHs (XoxF1 and MxaFI), that are involved in methylotrophy, and an ExaF-type Ln3+-dependent ethanol dehydrogenase, among six MDH-like genes in Methylobacterium aquaticum strain 22A. We also identified the causative mutations in MxbD, a sensor kinase necessary for mxaF expression and xoxF1 repression, for suppressive phenotypes in xoxF1 mutants defective in methanol growth even in the absence of Ln3+. Furthermore, we examined the phenotypes of a series of formaldehyde oxidation-pathway mutants (fae1, fae2, mch in the tetrahydromethanopterin (H4MPT) pathway and hgd in the glutathione-dependent formaldehyde dehydrogenase (GSH) pathway). We found that MxaF produces formaldehyde to a toxic level in the absence of the formaldehyde oxidation pathways and that either XoxF1 or ExaF can oxidize formaldehyde to alleviate formaldehyde toxicity in vivo. Furthermore, the GSH pathway has a supportive role for the net formaldehyde oxidation in addition to the H4MPT pathway that has primary importance. Studies on methylotrophy in Methylobacterium species have a long history, and this study provides further insights into genetic and physiological diversity and the differences in methylotrophy within the plant-colonizing methylotrophs.

    Original languageEnglish
    Article number822
    JournalMicroorganisms
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - Jun 2020

    Keywords

    • Lanthanide
    • Methanol dehydrogenase
    • Methylobacterium species
    • Methylotroph
    • XoxF

    ASJC Scopus subject areas

    • Microbiology
    • Microbiology (medical)
    • Virology

    Fingerprint

    Dive into the research topics of 'Lanthanide-dependent methanol and formaldehyde oxidation in methylobacterium aquaticum strain 22A'. Together they form a unique fingerprint.

    Cite this