Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis

Masataka Shinoda, Jun Ichi Haruta, Mitsune Tanimoto, Takafumi Ando, Takehiko Hosokawa, Kenji Ina, Kazuo Kusugami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Lymphokine-activated killer (LAK) cell activity and messenger RNA (mRNA) expression for interleukin-2 (IL-2) were analyzed using lamina propria mononuclear cells (LPMC) in inflammatory bowel disease patients. Compared with control LPMC, Crohn's disease (CD) LPMC exhibited significantly higher levels of LAK cell activity, whereas ulcerative colitis (UC) cells showed significantly lower levels of cytolytic activity with a difference in the frequency of CD3+ CD56+ and CD3-, CD56+ LAK precursor cells. After incubation with IL-2, the proportion of CD3+ CD56+ lymphocytes continued to be higher in CD cultures and substantially lower in UC cultures. Freshly isolated CD LPMC exhibited significantly higher levels of IL-2 mRNA than controls. However, no significant difference was observed between UC and control cells. The level of IL-2 expression or responsiveness to IL-2 may be responsible for different mucosal immune reactivity between CD and UC patients.

Original languageEnglish
Pages (from-to)679-685
Number of pages7
JournalInternal Medicine
Volume35
Issue number9
Publication statusPublished - Sep 1996
Externally publishedYes

Fingerprint

Ulcerative Colitis
Crohn Disease
Interleukin-2
Mucous Membrane
Lymphokine-Activated Killer Cells
Messenger RNA
Inflammatory Bowel Diseases
Lymphocytes

Keywords

  • Inflammatory bowel disease
  • Lymphokine-activated killer cell activity

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Shinoda, M., Haruta, J. I., Tanimoto, M., Ando, T., Hosokawa, T., Ina, K., & Kusugami, K. (1996). Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis. Internal Medicine, 35(9), 679-685.

Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis. / Shinoda, Masataka; Haruta, Jun Ichi; Tanimoto, Mitsune; Ando, Takafumi; Hosokawa, Takehiko; Ina, Kenji; Kusugami, Kazuo.

In: Internal Medicine, Vol. 35, No. 9, 09.1996, p. 679-685.

Research output: Contribution to journalArticle

Shinoda, M, Haruta, JI, Tanimoto, M, Ando, T, Hosokawa, T, Ina, K & Kusugami, K 1996, 'Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis', Internal Medicine, vol. 35, no. 9, pp. 679-685.
Shinoda M, Haruta JI, Tanimoto M, Ando T, Hosokawa T, Ina K et al. Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis. Internal Medicine. 1996 Sep;35(9):679-685.
Shinoda, Masataka ; Haruta, Jun Ichi ; Tanimoto, Mitsune ; Ando, Takafumi ; Hosokawa, Takehiko ; Ina, Kenji ; Kusugami, Kazuo. / Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis. In: Internal Medicine. 1996 ; Vol. 35, No. 9. pp. 679-685.
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