Lactoferrin markedly inhibits hepatitis C virus infection in cultured human hepatocytes

Masanori Ikeda, Kazuo Sugiyama, Torahiko Tanaka, Katsuaki Tanaka, Hisahiko Sekihara, Kunitada Shimotohno, Nobuyuki Kato

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

We found that bovine lactoferrin (bLF), a milk protein belonging to the iron transporter family, effectively prevented hepatitis C virus (HCV) infection in cultured human hepatocytes (PH5CH8), a cell line susceptible to HCV infection and supportive of HCV replication. Because preincubation of HCV with bLF was required to prevent the infection of HCV to the cells, and preincubation of bLF with the cells showed no inhibitory effect on HCV infection, we demonstrated that the anti-HCV activity of bLF was due to the interaction of bLF with HCV, but not due to the interaction of bLF with the cells. We further found that human lactoferrin also had anti-HCV activity, but bovine transferrin, the other member of the iron transporter family, did not have anti-HCV activity. Our findings suggest that lactoferrin is one of candidates for an anti-HCV reagent that will be well-tolerated and effective in the treatment of patients with chronic hepatitis.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume245
Issue number2
DOIs
Publication statusPublished - Apr 17 1998
Externally publishedYes

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Lactoferrin
Virus Diseases
Viruses
Hepacivirus
Hepatocytes
Iron
Milk Proteins
Chronic Hepatitis
Transferrin
Virus Replication
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lactoferrin markedly inhibits hepatitis C virus infection in cultured human hepatocytes. / Ikeda, Masanori; Sugiyama, Kazuo; Tanaka, Torahiko; Tanaka, Katsuaki; Sekihara, Hisahiko; Shimotohno, Kunitada; Kato, Nobuyuki.

In: Biochemical and Biophysical Research Communications, Vol. 245, No. 2, 17.04.1998, p. 549-553.

Research output: Contribution to journalArticle

Ikeda, Masanori ; Sugiyama, Kazuo ; Tanaka, Torahiko ; Tanaka, Katsuaki ; Sekihara, Hisahiko ; Shimotohno, Kunitada ; Kato, Nobuyuki. / Lactoferrin markedly inhibits hepatitis C virus infection in cultured human hepatocytes. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 245, No. 2. pp. 549-553.
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