TY - JOUR
T1 - Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds
AU - Morita, Hidetoshi
AU - Shiratori, Chiharu
AU - Murakami, Masaru
AU - Takami, Hideto
AU - Kato, Yukio
AU - Endo, Akihito
AU - Nakajima, Fumihiko
AU - Takagi, Misako
AU - Akita, Hiroaki
AU - Okada, Sanae
AU - Masaoka, Toshio
PY - 2007/12
Y1 - 2007/12
N2 - Two strains, KBL13T and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13T and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA-DNA hybridization, DNA G + C content and biochemical characterization indicated that these two strains, KBL13T and GBL13, belong to the same species. In the representative strain, KBL13T, the DNA G + C content was 34.3 mol%. Lactobacillus salivarius JCM 1231T (= ATCC 11741T; AF089108) is the type strain most closely related to the strain KBL13T as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0% (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA-DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13T represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13T (=JCM 14209T =DSM 18933T).
AB - Two strains, KBL13T and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13T and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA-DNA hybridization, DNA G + C content and biochemical characterization indicated that these two strains, KBL13T and GBL13, belong to the same species. In the representative strain, KBL13T, the DNA G + C content was 34.3 mol%. Lactobacillus salivarius JCM 1231T (= ATCC 11741T; AF089108) is the type strain most closely related to the strain KBL13T as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0% (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA-DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13T represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13T (=JCM 14209T =DSM 18933T).
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U2 - 10.1099/ijs.0.65135-0
DO - 10.1099/ijs.0.65135-0
M3 - Article
C2 - 18048734
AN - SCOPUS:37449022453
SN - 1466-5026
VL - 57
SP - 2836
EP - 2839
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
IS - 12
ER -