Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds

Hidetoshi Morita, Chiharu Shiratori, Masaru Murakami, Hideto Takami, Yukio Kato, Akihito Endo, Fumihiko Nakajima, Misako Takagi, Hiroaki Akita, Sanae Okada, Toshio Masaoka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Two strains, KBL13T and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13T and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA-DNA hybridization, DNA G + C content and biochemical characterization indicated that these two strains, KBL13T and GBL13, belong to the same species. In the representative strain, KBL13T, the DNA G + C content was 34.3 mol%. Lactobacillus salivarius JCM 1231T (= ATCC 11741T; AF089108) is the type strain most closely related to the strain KBL13T as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0% (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA-DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13T represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13T (=JCM 14209T =DSM 18933T).

Original languageEnglish
Pages (from-to)2836-2839
Number of pages4
JournalInternational journal of systematic and evolutionary microbiology
Volume57
Issue number12
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Morita, H., Shiratori, C., Murakami, M., Takami, H., Kato, Y., Endo, A., Nakajima, F., Takagi, M., Akita, H., Okada, S., & Masaoka, T. (2007). Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds. International journal of systematic and evolutionary microbiology, 57(12), 2836-2839. https://doi.org/10.1099/ijs.0.65135-0