Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds

Hidetoshi Morita, Akiyo Nakano, Mitsuharu Shimazu, Hidehiro Toh, Fumihiko Nakajima, Masahiro Nagayama, Shin Hisamatsu, Yukio Kato, Misako Takagi, Hideto Takami, Hiroaki Akita, Minoru Matsumoto, Toshio Masaoka, Masaru Murakami

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To detect the predominant lactobacilli in the intestinal flora of healthy thoroughbreds, we isolated lactobacilli from the feces of nine thoroughbreds (five males and four females; 0-15-year-old). The isolated lactobacilli comprise 17 species (37 strains), and they were classified into five groups: Lactobacillus salivarius (6 species), L. reuteri (6 species), Lactobacillus delbrueckii (3 species), L. buchneri (1 species) and L. vitulinus (1 species). On the basis of 16S rRNA gene sequences, we identified 3 other phylogenetic relatives belonging to the genus Lactobacillus. These results suggest that the intestinal flora of thoroughbreds may comprise many species of the genus Lactobacillus. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the 340-bp fragments of the 16S rRNA genes from the same nine fecal samples showed that L. hayakitensis, L. equigenerosi and L. equi are contained in all the samples, suggesting that these species are predominant lactobacilli in the intestinal flora of thoroughbreds.

Original languageEnglish
Pages (from-to)339-346
Number of pages8
JournalAnimal Science Journal
Volume80
Issue number3
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint

Lactobacillus equi
Lactobacillus
intestinal microorganisms
rRNA Genes
Kandleria vitulina
ribosomal RNA
Lactobacillus delbrueckii
Lactobacillus salivarius
Lactobacillus buchneri
Lactobacillus reuteri
Denaturing Gradient Gel Electrophoresis
Equus
denaturing gradient gel electrophoresis
Feces
Gastrointestinal Microbiome
feces
polymerase chain reaction
nucleotide sequences
sampling
Polymerase Chain Reaction

Keywords

  • Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)
  • Predominant lactobacilli
  • Thoroughbred intestinal flora

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds. / Morita, Hidetoshi; Nakano, Akiyo; Shimazu, Mitsuharu; Toh, Hidehiro; Nakajima, Fumihiko; Nagayama, Masahiro; Hisamatsu, Shin; Kato, Yukio; Takagi, Misako; Takami, Hideto; Akita, Hiroaki; Matsumoto, Minoru; Masaoka, Toshio; Murakami, Masaru.

In: Animal Science Journal, Vol. 80, No. 3, 06.2009, p. 339-346.

Research output: Contribution to journalArticle

Morita, H, Nakano, A, Shimazu, M, Toh, H, Nakajima, F, Nagayama, M, Hisamatsu, S, Kato, Y, Takagi, M, Takami, H, Akita, H, Matsumoto, M, Masaoka, T & Murakami, M 2009, 'Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds', Animal Science Journal, vol. 80, no. 3, pp. 339-346. https://doi.org/10.1111/j.1740-0929.2009.00633.x
Morita, Hidetoshi ; Nakano, Akiyo ; Shimazu, Mitsuharu ; Toh, Hidehiro ; Nakajima, Fumihiko ; Nagayama, Masahiro ; Hisamatsu, Shin ; Kato, Yukio ; Takagi, Misako ; Takami, Hideto ; Akita, Hiroaki ; Matsumoto, Minoru ; Masaoka, Toshio ; Murakami, Masaru. / Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds. In: Animal Science Journal. 2009 ; Vol. 80, No. 3. pp. 339-346.
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