Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice

Ichiro Nakamura, Naoyuki Takahashi, Nobuyuki Udagawa, Yoshinori Moriyama, Takahide Kurokawa, Eijiro Jimi, Takahisa Sasaki, Tatsuo Suda

Research output: Contribution to journalArticle

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Abstract

We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+-ATPase (V-ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V-ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell-derived osteoclast-like cells (OCLs), immunoreactivity for V-ATPase was detected in association with Triton X-100-insoluble cellular structure, but not in oc/oc spleen cell-derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V-ATPase and cytoskeleton in osteoclasts.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalFEBS Letters
Volume401
Issue number2-3
DOIs
Publication statusPublished - Jan 20 1997
Externally publishedYes

Fingerprint

Vacuolar Proton-Translocating ATPases
Osteoclasts
Cytoskeleton
Adenosine Triphosphatases
Protons
Association reactions
Spleen
Osteosclerosis
Proximal Kidney Tubule
Octoxynol
Cellular Structures
Bone Resorption
Bone
Cytoplasm
Membranes

Keywords

  • oc/oc
  • Osteosclerosis
  • Renal proximal convoluted tubule
  • Ruffled border
  • Vacuolar proton ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nakamura, I., Takahashi, N., Udagawa, N., Moriyama, Y., Kurokawa, T., Jimi, E., ... Suda, T. (1997). Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice. FEBS Letters, 401(2-3), 207-212. https://doi.org/10.1016/S0014-5793(96)01454-8

Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice. / Nakamura, Ichiro; Takahashi, Naoyuki; Udagawa, Nobuyuki; Moriyama, Yoshinori; Kurokawa, Takahide; Jimi, Eijiro; Sasaki, Takahisa; Suda, Tatsuo.

In: FEBS Letters, Vol. 401, No. 2-3, 20.01.1997, p. 207-212.

Research output: Contribution to journalArticle

Nakamura, I, Takahashi, N, Udagawa, N, Moriyama, Y, Kurokawa, T, Jimi, E, Sasaki, T & Suda, T 1997, 'Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice', FEBS Letters, vol. 401, no. 2-3, pp. 207-212. https://doi.org/10.1016/S0014-5793(96)01454-8
Nakamura, Ichiro ; Takahashi, Naoyuki ; Udagawa, Nobuyuki ; Moriyama, Yoshinori ; Kurokawa, Takahide ; Jimi, Eijiro ; Sasaki, Takahisa ; Suda, Tatsuo. / Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice. In: FEBS Letters. 1997 ; Vol. 401, No. 2-3. pp. 207-212.
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