Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice

Ichiro Nakamura, Naoyuki Takahashi, Nobuyuki Udagawa, Yoshinori Moriyama, Takahide Kurokawa, Eijiro Jimi, Takahisa Sasaki, Tatsuo Suda

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+-ATPase (V-ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V-ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell-derived osteoclast-like cells (OCLs), immunoreactivity for V-ATPase was detected in association with Triton X-100-insoluble cellular structure, but not in oc/oc spleen cell-derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V-ATPase and cytoskeleton in osteoclasts.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalFEBS Letters
Volume401
Issue number2-3
DOIs
Publication statusPublished - Jan 20 1997

Keywords

  • Osteosclerosis
  • Renal proximal convoluted tubule
  • Ruffled border
  • Vacuolar proton ATPase
  • oc/oc

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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