Abstract
Histamine plays an important role in the regulation of gastric acid secretion; however, its role in maintenance of gastric morphology remains unclear. To clarify the necessity of histamine for gastric mucosal development and maintenance, we evaluated two different kinds of mice that lacked either mast cells (one of the gastric histamine-producing cell types) or histidine decarboxylase (HDC; a histamine-synthesizing enzyme). Measurements of stomach weight, intragastric pH, mucosal histamine levels, as well as serum gastrin and albumin levels were performed in mice. Gastric mucosal appearance was examined by immunohistochemical techniques. Although gastric mucosal histamine levels in mast cell-deficient mice were half of those observed in the wild-type mice, intragastric pH, serum gastrin levels, and gastric morphology at 12 mo were unchanged compared with the wild-type mice. In contrast, HDC-deficient mice possessed no detectable gastric histamine, but did exhibit hypergastrinemia, as well as marked increases in intragastric pH and stomach weight compared with the wild-type mice. Histological analysis revealed that 9-mo-old HDC-deficient mice demonstrated hyperplasia in the oxyntic glandular base region, as well as increased numbers of parietal and enterochromaffin-like cells. These results indicate that enterochromaffin-like cell-derived histamine is potentially involved in gastric mucosal morphology regulation.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 287 |
| Issue number | 5 50-5 |
| DOIs | |
| Publication status | Published - Nov 2004 |
| Externally published | Yes |
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Keywords
- Enterochromaffin-like cell
- Hypergastrinemia
- Parietal cell
ASJC Scopus subject areas
- Gastroenterology
- Physiology
Cite this
Lack of histamine alters gastric mucosal morphology : Comparison of histidine decarboxylase-deficient and mast cell-deficient mice. / Nakamura, Eiji; Kataoka, Takashi; Furutani, Kazuharu; Jimbo, Keisuke; Aihara, Takeshi; Tanaka, Satoshi; Ichikawa, Atsushi; Ohtsu, Hiroshi; Okabe, Susumu.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 287, No. 5 50-5, 11.2004.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of histamine alters gastric mucosal morphology
T2 - Comparison of histidine decarboxylase-deficient and mast cell-deficient mice
AU - Nakamura, Eiji
AU - Kataoka, Takashi
AU - Furutani, Kazuharu
AU - Jimbo, Keisuke
AU - Aihara, Takeshi
AU - Tanaka, Satoshi
AU - Ichikawa, Atsushi
AU - Ohtsu, Hiroshi
AU - Okabe, Susumu
PY - 2004/11
Y1 - 2004/11
N2 - Histamine plays an important role in the regulation of gastric acid secretion; however, its role in maintenance of gastric morphology remains unclear. To clarify the necessity of histamine for gastric mucosal development and maintenance, we evaluated two different kinds of mice that lacked either mast cells (one of the gastric histamine-producing cell types) or histidine decarboxylase (HDC; a histamine-synthesizing enzyme). Measurements of stomach weight, intragastric pH, mucosal histamine levels, as well as serum gastrin and albumin levels were performed in mice. Gastric mucosal appearance was examined by immunohistochemical techniques. Although gastric mucosal histamine levels in mast cell-deficient mice were half of those observed in the wild-type mice, intragastric pH, serum gastrin levels, and gastric morphology at 12 mo were unchanged compared with the wild-type mice. In contrast, HDC-deficient mice possessed no detectable gastric histamine, but did exhibit hypergastrinemia, as well as marked increases in intragastric pH and stomach weight compared with the wild-type mice. Histological analysis revealed that 9-mo-old HDC-deficient mice demonstrated hyperplasia in the oxyntic glandular base region, as well as increased numbers of parietal and enterochromaffin-like cells. These results indicate that enterochromaffin-like cell-derived histamine is potentially involved in gastric mucosal morphology regulation.
AB - Histamine plays an important role in the regulation of gastric acid secretion; however, its role in maintenance of gastric morphology remains unclear. To clarify the necessity of histamine for gastric mucosal development and maintenance, we evaluated two different kinds of mice that lacked either mast cells (one of the gastric histamine-producing cell types) or histidine decarboxylase (HDC; a histamine-synthesizing enzyme). Measurements of stomach weight, intragastric pH, mucosal histamine levels, as well as serum gastrin and albumin levels were performed in mice. Gastric mucosal appearance was examined by immunohistochemical techniques. Although gastric mucosal histamine levels in mast cell-deficient mice were half of those observed in the wild-type mice, intragastric pH, serum gastrin levels, and gastric morphology at 12 mo were unchanged compared with the wild-type mice. In contrast, HDC-deficient mice possessed no detectable gastric histamine, but did exhibit hypergastrinemia, as well as marked increases in intragastric pH and stomach weight compared with the wild-type mice. Histological analysis revealed that 9-mo-old HDC-deficient mice demonstrated hyperplasia in the oxyntic glandular base region, as well as increased numbers of parietal and enterochromaffin-like cells. These results indicate that enterochromaffin-like cell-derived histamine is potentially involved in gastric mucosal morphology regulation.
KW - Enterochromaffin-like cell
KW - Hypergastrinemia
KW - Parietal cell
UR - http://www.scopus.com/inward/record.url?scp=7044220771&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7044220771&partnerID=8YFLogxK
U2 - 10.1152/ajpgi.00353.2003
DO - 10.1152/ajpgi.00353.2003
M3 - Article
C2 - 15271653
AN - SCOPUS:7044220771
VL - 287
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
SN - 0193-1857
IS - 5 50-5
ER -