Lack of endothelin ETB receptor binding and function in the rat with a mutant ETB receptor gene

Hideaki Karaki, Minori Mitsui-Saito, Misato Takimoto, Kyoko Oda, Toshikazu Okada, Tsuyoshi Ozaki, Tetsuo Kunieda

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Congenital aganglionosis rat is a mutant with an autosomal recessive gene (sl). Recent studies have revealed that the endothelin ETB receptor gene of sl/sl rat has a deletion of 301-bp region spanning exon 1 and intron 1 corresponding to the first and the second transmembrane domains of the receptor. In the present experiments, we examined the functions of ETB receptors in the sl/sl rats. In the membranes of cerebellum, heart, and lung of control (+/+ and sl/+) rats, ET-1 induced a monophasic, competitive displacement of [125I]ET-1 binding, whereas ET-3, IRL 1620, and BQ-123 showed biphasic displacement. In the membranes of sl/sl rats, in contrast, ET-1, BQ-123, ET-3, and IRL 1620 showed only monophasic displacement. Scatchard analysis revealed a single [125I]ET-3 binding site in the membrane of control heart but not in the sl/sl rat heart, and the specific binding sites for [125I]ET-1 in both control and sl/sl rat hearts. In the control rat aorta but not in the sl/sl rat aorta, ET-3 induced endothelium-dependent relaxation. These results suggest that sl/sl rats do not have functional ETB receptors.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume222
Issue number1
DOIs
Publication statusPublished - May 6 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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