Lack of direct involvement of 8-hydroxy-2′-deoxyguanosine in hypoxanthine-guanine phosphoribosyltransferase mutagenesis in V79 cells treated with N,N′-Bis(2-hydroxyperooxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylicdiimide (NP-III) or Riboflavin

Madoka Nakajima, Toru Takeuchi, Keiki Ogino, Kanehisa Morimoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The object of this study is to investigate the relationship between a typical product of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine (8OHdG), and mutagenesis in V79 cells through a molecular analysis of hypoxanthine-guanine phosphoribosyltransferase (hprt) gene mutants. We performed a direct sequencing analysis of the cDNA of mutants obtained after treatment with N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylic-diimide (NP-III) or riboflavin, each of which induces the formation of 8OHdG in cellular DNA upon UVA irradiation. The frequency of mutation after both treatments was no more than 2 to 5 times the control value. A considerable number of the mutants could not be amplified by RT-PCR, and this was also the case for the control mutants. Among the mutants analyzed, deletions and a TA→AT transversion occurred predominantly. The reasons for the weak association of induction of 8OHdG with frequency of mutation and the possible mechanism of oxidative-stress-derived mutagenesis are discussed.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalJapanese Journal of Cancer Research
Volume93
Issue number3
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Hypoxanthine Phosphoribosyltransferase
Riboflavin
Mutation Rate
Mutagenesis
DNA Damage
Oxidative Stress
Complementary DNA
Polymerase Chain Reaction
DNA
Genes
1,4,5,8-naphthalenetetracarboxylic diimide
8-oxo-7-hydrodeoxyguanosine

Keywords

  • 8-hydroxy-2′-deoxyguanosine
  • Deletion
  • hprt
  • Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Lack of direct involvement of 8-hydroxy-2′-deoxyguanosine in hypoxanthine-guanine phosphoribosyltransferase mutagenesis in V79 cells treated with N,N′-Bis(2-hydroxyperooxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylicdiimide (NP-III) or Riboflavin",
abstract = "The object of this study is to investigate the relationship between a typical product of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine (8OHdG), and mutagenesis in V79 cells through a molecular analysis of hypoxanthine-guanine phosphoribosyltransferase (hprt) gene mutants. We performed a direct sequencing analysis of the cDNA of mutants obtained after treatment with N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylic-diimide (NP-III) or riboflavin, each of which induces the formation of 8OHdG in cellular DNA upon UVA irradiation. The frequency of mutation after both treatments was no more than 2 to 5 times the control value. A considerable number of the mutants could not be amplified by RT-PCR, and this was also the case for the control mutants. Among the mutants analyzed, deletions and a TA→AT transversion occurred predominantly. The reasons for the weak association of induction of 8OHdG with frequency of mutation and the possible mechanism of oxidative-stress-derived mutagenesis are discussed.",
keywords = "8-hydroxy-2′-deoxyguanosine, Deletion, hprt, Mutation",
author = "Madoka Nakajima and Toru Takeuchi and Keiki Ogino and Kanehisa Morimoto",
year = "2002",
language = "English",
volume = "93",
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T1 - Lack of direct involvement of 8-hydroxy-2′-deoxyguanosine in hypoxanthine-guanine phosphoribosyltransferase mutagenesis in V79 cells treated with N,N′-Bis(2-hydroxyperooxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylicdiimide (NP-III) or Riboflavin

AU - Nakajima, Madoka

AU - Takeuchi, Toru

AU - Ogino, Keiki

AU - Morimoto, Kanehisa

PY - 2002

Y1 - 2002

N2 - The object of this study is to investigate the relationship between a typical product of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine (8OHdG), and mutagenesis in V79 cells through a molecular analysis of hypoxanthine-guanine phosphoribosyltransferase (hprt) gene mutants. We performed a direct sequencing analysis of the cDNA of mutants obtained after treatment with N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylic-diimide (NP-III) or riboflavin, each of which induces the formation of 8OHdG in cellular DNA upon UVA irradiation. The frequency of mutation after both treatments was no more than 2 to 5 times the control value. A considerable number of the mutants could not be amplified by RT-PCR, and this was also the case for the control mutants. Among the mutants analyzed, deletions and a TA→AT transversion occurred predominantly. The reasons for the weak association of induction of 8OHdG with frequency of mutation and the possible mechanism of oxidative-stress-derived mutagenesis are discussed.

AB - The object of this study is to investigate the relationship between a typical product of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine (8OHdG), and mutagenesis in V79 cells through a molecular analysis of hypoxanthine-guanine phosphoribosyltransferase (hprt) gene mutants. We performed a direct sequencing analysis of the cDNA of mutants obtained after treatment with N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8- naphthalenetetracarboxylic-diimide (NP-III) or riboflavin, each of which induces the formation of 8OHdG in cellular DNA upon UVA irradiation. The frequency of mutation after both treatments was no more than 2 to 5 times the control value. A considerable number of the mutants could not be amplified by RT-PCR, and this was also the case for the control mutants. Among the mutants analyzed, deletions and a TA→AT transversion occurred predominantly. The reasons for the weak association of induction of 8OHdG with frequency of mutation and the possible mechanism of oxidative-stress-derived mutagenesis are discussed.

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