Lack of B-RAF mutations in head and neck squamous cell carcinoma

M. Al Sheikh Ali, Mehmet Gunduz, E. Gunduz, R. Tamamura, L. Beder, S. Tominaga, T. Onoda, N. Yamanaka, R. Grenman, K. Shimizu, N. Nagai, H. Nagatsuka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

B-RAF is one of the most commonly mutated oncogenes in human cancer. However, the mutation status of B-RAF has not been established completely in HNSCC. We have analysed the mutation status of the kinase domain of the B-RAF gene (exons 11 and 15) in 91 Japanese HNSCC patients as well as 12 HNSCC cell lines. DNA was extracted and amplified by PCR. Mutations were then analysed by SSCP mutation detection method. Since V600EB-RAF constitutes 90% of the mutations identified in B-RAF in human cancers, we also used MASA analysis to specifically detect this mutation in exon 15 of B-RAF. Using both methods, no mutation was found in both exon 11 and 15 in all patients and cell lines. Mutations are absent or rare in the kinase domain of B-RAF in Japanese HNSCC. However, more studies are still needed to determine its usefulness as a target for molecular therapy in these patients.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalFolia Biologica
Volume54
Issue number5
Publication statusPublished - Jan 1 2008

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Molecular Biology
  • Genetics
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Al Sheikh Ali, M., Gunduz, M., Gunduz, E., Tamamura, R., Beder, L., Tominaga, S., Onoda, T., Yamanaka, N., Grenman, R., Shimizu, K., Nagai, N., & Nagatsuka, H. (2008). Lack of B-RAF mutations in head and neck squamous cell carcinoma. Folia Biologica, 54(5), 157-161.