Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media

Muhammad Moniruzzaman, Yoichiro Nao, Sekhar Bhattacharjee, Tsutomu Ono

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) [C2mim][PF6] (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions.

Original languageEnglish
Title of host publicationProcess and Advanced Materials Engineering
EditorsIqbal Ahmed
PublisherTrans Tech Publications Ltd
Pages333-336
Number of pages4
ISBN (Electronic)9783038351818
DOIs
Publication statusPublished - Jan 1 2014
Event3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014 - Kuala Lumpur, Malaysia
Duration: Jun 3 2014Jun 5 2014

Publication series

NameApplied Mechanics and Materials
Volume625
ISSN (Print)1660-9336
ISSN (Electronic)1662-7482

Other

Other3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014
CountryMalaysia
CityKuala Lumpur
Period6/3/146/5/14

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Keywords

  • Enzyme activity and stability
  • Enzyme immobilization
  • Ionic liquids
  • Laccase poly (ethylene glycol)-block-polylactide emulsions

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Moniruzzaman, M., Nao, Y., Bhattacharjee, S., & Ono, T. (2014). Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media. In I. Ahmed (Ed.), Process and Advanced Materials Engineering (pp. 333-336). (Applied Mechanics and Materials; Vol. 625). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/AMM.625.333