Label free immune assay using terahertz chemical microscope

Toshihiko Kiwa, Akira Tenma, Shinji Takahashi, Kenji Sakai, Keiji Tsukada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. The sensing plate can emit the terahertz pulses by femtosecond laser pulses and the amplitude of the terahertz pulses can be related to the chemical or electric potential of the sensing plate surface at where the laser pulses are irradiated. Thus the potential distribution can be visualized as the map of the terahertz pulses amplitude. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The terahertz pulses were enhanced at where anti-IgG was combined with IgG on the sensing plate and the distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images. The amplitude of terahertz pulses as a function of the concentration of anti-IgG was also investigated.

Original languageEnglish
Pages (from-to)8-11
Number of pages4
JournalSensors and Actuators, B: Chemical
Volume187
DOIs
Publication statusPublished - 2013

Fingerprint

Antibodies
Labels
Laser pulses
Assays
Microscopes
Immunoglobulin G
microscopes
Chemical potential
Antigens
pulses
Ultrashort pulses
Demonstrations
antibodies
Silicon
mice
Electric potential
antigens
pulse amplitude
markers
lasers

Keywords

  • Bio-sensing
  • Femtosecond laser
  • Immunoglobulin G
  • Terahertz

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Label free immune assay using terahertz chemical microscope. / Kiwa, Toshihiko; Tenma, Akira; Takahashi, Shinji; Sakai, Kenji; Tsukada, Keiji.

In: Sensors and Actuators, B: Chemical, Vol. 187, 2013, p. 8-11.

Research output: Contribution to journalArticle

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