@article{ff142c267f784d4b893ed08ca8509f06,
title = "Label free immune assay using terahertz chemical microscope",
abstract = "A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. The sensing plate can emit the terahertz pulses by femtosecond laser pulses and the amplitude of the terahertz pulses can be related to the chemical or electric potential of the sensing plate surface at where the laser pulses are irradiated. Thus the potential distribution can be visualized as the map of the terahertz pulses amplitude. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The terahertz pulses were enhanced at where anti-IgG was combined with IgG on the sensing plate and the distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images. The amplitude of terahertz pulses as a function of the concentration of anti-IgG was also investigated.",
keywords = "Bio-sensing, Femtosecond laser, Immunoglobulin G, Terahertz",
author = "Toshihiko Kiwa and Akira Tenma and Shinji Takahashi and Kenji Sakai and Keiji Tsukada",
note = "Funding Information: As the first demonstration of the label free immune assay using TCM, reactions of anti-IgG to IgG was observed. The amplitude of the THz pulses were changed at where the anti-IgG and IgG bindings were formed at the sensing plates which may result in the change in the potential on the sensing plate. The results also indicate that the change in the amplitude could be related to the concentration of the anti-IgG reacted to the IgG, which suggests that that TCM can be one of useful candidate for a label-free immunoassay system. Toshihiko Kiwa was received the B.E., M.E. and PhD degree from Osaka University in 1998, 2000 and 2003, respectively. After that, he worked for 1-year as a JSPS fellow at the Research Center for Superconductor Photonics, Osaka University, where he was involved in the development of terahertz and superconductor devices. Currently, he is a lecturer of Department of Electrical and Electronic Engineering, Okayama University. His research interests include chemical sensors, magneto metric sensors, and terahertz sensing devices. Akira Tenma was received the B.E., and M.E. degree from Okayama University in 2011, and now he is with Murata Manufacturing Co., Ltd. Shinji Takahashi was received the B.E. degree in 2012. He is the master course student of Okayama University and studies terahertz technologies. Kenji Sakai was received PhD degree from Doshisha University in 2010. He was a Research Fellow of the Japan Society for the Promotion of Science from 2009 to 2010. He is presently an Assistant Professor of Department of Electrical and Electronic Engineering, Okayama University. He is now involved in the research of superconducting sensor devices and their applications, non-destructive evaluation system, and gas sensor. Keiji Tsukada was received Dr. Eng. and the PhD degrees from Tsukuba University in 1990, and 2001, respectively. He joined the Central Research Laboratory, Hitachi Ltd. in 1982, where he was involved in the study of integrated solid-state chemical sensor for blood analyses. He was with the Superconducting Sensor Laboratory from 1991 to 1996. He was involved in the research and development of SQUID's and multichannel SQUID system. He was with the Central Research Laboratory, Hitachi Ltd. from 1996 to 2003. He was a Project Leader of the SQUID application research group. He is presently a Professor of Department of Electrical and Electronic Engineering, Okayama University. He is involved in the research of gas chemical sensor and superconducting sensor devices, and their applications. ",
year = "2013",
doi = "10.1016/j.snb.2012.08.051",
language = "English",
volume = "187",
pages = "8--11",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
}