Abstract
A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. The sensing plate can emit the terahertz pulses by femtosecond laser pulses and the amplitude of the terahertz pulses can be related to the chemical or electric potential of the sensing plate surface at where the laser pulses are irradiated. Thus the potential distribution can be visualized as the map of the terahertz pulses amplitude. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The terahertz pulses were enhanced at where anti-IgG was combined with IgG on the sensing plate and the distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images. The amplitude of terahertz pulses as a function of the concentration of anti-IgG was also investigated.
| Original language | English |
|---|---|
| Pages (from-to) | 8-11 |
| Number of pages | 4 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 187 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- Bio-sensing
- Femtosecond laser
- Immunoglobulin G
- Terahertz
ASJC Scopus subject areas
- Instrumentation
- Materials Chemistry
- Surfaces, Coatings and Films
- Metals and Alloys
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Electrical and Electronic Engineering
Cite this
Label free immune assay using terahertz chemical microscope. / Kiwa, Toshihiko; Tenma, Akira; Takahashi, Shinji; Sakai, Kenji; Tsukada, Keiji.
In: Sensors and Actuators, B: Chemical, Vol. 187, 2013, p. 8-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Label free immune assay using terahertz chemical microscope
AU - Kiwa, Toshihiko
AU - Tenma, Akira
AU - Takahashi, Shinji
AU - Sakai, Kenji
AU - Tsukada, Keiji
PY - 2013
Y1 - 2013
N2 - A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. The sensing plate can emit the terahertz pulses by femtosecond laser pulses and the amplitude of the terahertz pulses can be related to the chemical or electric potential of the sensing plate surface at where the laser pulses are irradiated. Thus the potential distribution can be visualized as the map of the terahertz pulses amplitude. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The terahertz pulses were enhanced at where anti-IgG was combined with IgG on the sensing plate and the distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images. The amplitude of terahertz pulses as a function of the concentration of anti-IgG was also investigated.
AB - A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of the antibody-antigen bindings on the silicon based sensing plate without any labels and/or markers on the antibody. The sensing plate can emit the terahertz pulses by femtosecond laser pulses and the amplitude of the terahertz pulses can be related to the chemical or electric potential of the sensing plate surface at where the laser pulses are irradiated. Thus the potential distribution can be visualized as the map of the terahertz pulses amplitude. As the first demonstration of the label free immune assay, the mouse immunoglobulin G (IgG) was immobilized on the half part of the sensing plate and the mouse anti-IgG was combined with the IgG. The terahertz pulses were enhanced at where anti-IgG was combined with IgG on the sensing plate and the distributions of the IgG and anti-IgG bindings on the sensing plate were clearly seen as the TCM images. The amplitude of terahertz pulses as a function of the concentration of anti-IgG was also investigated.
KW - Bio-sensing
KW - Femtosecond laser
KW - Immunoglobulin G
KW - Terahertz
UR - http://www.scopus.com/inward/record.url?scp=84885471190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885471190&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2012.08.051
DO - 10.1016/j.snb.2012.08.051
M3 - Article
VL - 187
SP - 8
EP - 11
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -