Label-free detection of proteins using a terahertz chemical microscopy

M. Ogawa, A. Nakamura, K. Omura, Kenji Sakai, Toshihiko Kiwa, Keiji Tsukada

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A terahertz chemical microscopy (TCM) has been proposed and developed to visualize the distribution of chemical reactions such as antigen-antibody reaction on the sensing plate without any labels on the analyte. The TCM utilize the semiconductor-based device named a sensing plate. The sensing plate can convert the shift of the chemical potential in the magnitude on the surface of sensing plate into the amplitude of generated terahertz (THz) in the sensing plate. Thus, it enables to get images of the potential distribution on the surface of the sensing plate as the distribution of THz amplitude. Here, we demonstrate the detection of the immune reactions between the mouse immunoglobulin G (IgG) and the anti-mouse immunoglobulin G (anti-IgG). IgG was immobilized on the sensing plate and reacted with anti-IgG in the solutions with various concentrations. We also fabricated the sample wells on the sensing plate and quantitative evaluation of immune reactions were demonstrated with the anti-IgG concentration of between 5 nM and 100 nM. As a result, the change in the THz amplitude during the reaction showed the proportional relation with the concentration of reacted anti-IgG.

Original languageEnglish
Title of host publication2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Print)9781467352253
DOIs
Publication statusPublished - Oct 17 2014
Event31st General Assembly and Scientific Symposium of the International Union of Radio Science, URSI GASS 2014 - Beijing, China
Duration: Aug 16 2014Aug 23 2014

Other

Other31st General Assembly and Scientific Symposium of the International Union of Radio Science, URSI GASS 2014
CountryChina
CityBeijing
Period8/16/148/23/14

Fingerprint

Antigen-antibody reactions
Labels
Microscopic examination
Proteins
Chemical potential
Chemical reactions
Semiconductor materials

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Computer Networks and Communications

Cite this

Ogawa, M., Nakamura, A., Omura, K., Sakai, K., Kiwa, T., & Tsukada, K. (2014). Label-free detection of proteins using a terahertz chemical microscopy. In 2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014 [6930087] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/URSIGASS.2014.6930087

Label-free detection of proteins using a terahertz chemical microscopy. / Ogawa, M.; Nakamura, A.; Omura, K.; Sakai, Kenji; Kiwa, Toshihiko; Tsukada, Keiji.

2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. 6930087.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogawa, M, Nakamura, A, Omura, K, Sakai, K, Kiwa, T & Tsukada, K 2014, Label-free detection of proteins using a terahertz chemical microscopy. in 2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014., 6930087, Institute of Electrical and Electronics Engineers Inc., 31st General Assembly and Scientific Symposium of the International Union of Radio Science, URSI GASS 2014, Beijing, China, 8/16/14. https://doi.org/10.1109/URSIGASS.2014.6930087
Ogawa M, Nakamura A, Omura K, Sakai K, Kiwa T, Tsukada K. Label-free detection of proteins using a terahertz chemical microscopy. In 2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014. Institute of Electrical and Electronics Engineers Inc. 2014. 6930087 https://doi.org/10.1109/URSIGASS.2014.6930087
Ogawa, M. ; Nakamura, A. ; Omura, K. ; Sakai, Kenji ; Kiwa, Toshihiko ; Tsukada, Keiji. / Label-free detection of proteins using a terahertz chemical microscopy. 2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014. Institute of Electrical and Electronics Engineers Inc., 2014.
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